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Product Info Summary
| SKU: | A17853-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PLAC9 Antibody Picoband®
SKU/Catalog Number
A17853-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PLAC9 Antibody Picoband® catalog # A17853-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLAC9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17853-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PLAC9 recombinant protein (Position: R30-L81).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A17853-1 is reactive to PLAC9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
24145 MW
Background of PLAC9
By microarray assay to search for mouse genes enriched in placenta, Plac9 was identified as a placenta-enriched gene expressed in mouse 16-cell morula cDNA. By database analysis, human PLAC9, which encodes a deduced 97-amino acid protein, was identified. The deduced 108-amino acid mouse protein has a predicted signal peptide, a coiled-coil domain, and shares 73% identity with human PLAC9. Northern blot analysis of mouse placenta and embryo RNA at 12.5 and 18.5 days postcoitum (dpc) detected predominant expression in placenta.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17853-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PLAC9 using anti-PLAC9 antibody (A17853-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAC9 antigen affinity purified polyclonal antibody (Catalog # A17853-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAC9 at approximately 17KD. The expected band size for PLAC9 is at 10KD.
Protein Target Info & Infographic
Gene/Protein Information For PLAC9 (Source: Uniprot.org, NCBI)
Gene Name
PLAC9
Full Name
Placenta-specific protein 9
Weight
24145 MW
Superfamily
PLAC9 family
Alternative Names
Placenta-specific protein 9; PLAC9 PLAC9 placenta associated 9 placenta-specific protein 9|placenta specific 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLAC9, check out the PLAC9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLAC9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLAC9 Antibody Picoband® (A17853-1)
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Customer Q&As
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3 Customer Q&As for Anti-PLAC9 Antibody Picoband®
Question
We are currently using anti-PLAC9 antibody A17853-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
D. Krishna
Verified customer
Asked: 2020-05-06
Answer
The anti-PLAC9 antibody (A17853-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
Is there a BSA free version of anti-PLAC9 antibody A17853-1 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PLAC9 antibody A17853-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
I would like to test anti-PLAC9 antibody A17853-1 on human layer of synovial tissue for research purposes, then I may be interested in using anti-PLAC9 antibody A17853-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The products we sell, including anti-PLAC9 antibody A17853-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-24
