Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody

LPAR1/LPA1/EDG-2 antibody

Boster Bio Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody catalog # M02782. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Product Info Summary

SKU: M02782
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IHC, WB

Product Name

Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody

View all LPAR1/LPA1/EDG-2 Antibodies

SKU/Catalog Number

M02782

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody catalog # M02782. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02782)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACGE-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human EDG2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02782 is reactive to LPAR1 in Human

Applications

M02782 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

70 kDa, 90 kDa

Calculated molecular weight

98399 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For LPAR1 (Source: Uniprot.org, NCBI)

Gene Name

LPAR1

Full Name

Lysophosphatidic acid receptor 1

Weight

98399 MW

Superfamily

G-protein coupled receptor 1 family

Alternative Names

EDG2; edg-2; EDG2Lysophosphatidic acid receptor Edg-2; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,; GPCR26; GPR26; LPA receptor 1; LPA1; LPA-1; LPA1vzg-1; LPAR1; lysophosphatidic acid receptor 1; Mrec1.3; rec.1.3; ventricular zone gene 1; VZG1; vzg-1 LPAR1 EDG2, Gpcr26, LPA1, Mrec1.3, VZG1, edg-2, rec.1.3, vzg-1 lysophosphatidic acid receptor 1 lysophosphatidic acid receptor 1|LPA receptor 1|LPA-1|endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2|lysophosphatidic acid receptor Edg-2|ventricular zone gene 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LPAR1, check out the LPAR1 Infographic

LPAR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LPAR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M02782

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

9 Customer Q&As for Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody

Question

Is M02782 a recombinant antibody? What is the expression system and the transfection method?

Verified customer

Asked: 2021-01-12

Answer

The Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (M02782) is a recombinant antibody. Expression system is plasmid transfection with 293F without using any viral particles.

Boster Scientific Support

Answered: 2021-01-12

Question

What sample target on Western Blot application was tested with M02782?

Verified customer

Asked: 2020-01-03

Answer

Western blot analysis of EDG2 expression in A375 cell lysate.

Boster Scientific Support

Answered: 2020-01-16

Question

I see that the anti-EDG2 Monoclonal antibody M02782 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-21

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-EDG2 Monoclonal antibody M02782. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-19

Question

Will M02782 anti-EDG2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

As indicated on the product datasheet, M02782 anti-EDG2 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-21

Question

We are currently using anti-EDG2 Monoclonal antibody M02782 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

J. Jha

Verified customer

Asked: 2016-12-13

Answer

The anti-EDG2 Monoclonal antibody (M02782) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-12-13

Question

Does anti-EDG2 Monoclonal antibody M02782 work for IP with liver?

Z. Krishna

Verified customer

Asked: 2014-10-08

Answer

According to the expression profile of liver, LPAR1 is highly expressed in liver. So, it is likely that anti-EDG2 Monoclonal antibody M02782 will work for IP with liver.

Boster Scientific Support

Answered: 2014-10-08

Question

I was wanting to use your anti-EDG2 Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Z. Johnson

Verified customer

Asked: 2013-07-31

Answer

As indicated on the product datasheet, M02782 anti-EDG2 Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-07-31

Question

Does anti-EDG2 Monoclonal antibody M02782 work on monkey WB with liver?

N. Johnson

Verified customer

Asked: 2013-06-05

Answer

Our lab technicians have not validated anti-EDG2 Monoclonal antibody M02782 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EDG2 Monoclonal antibody M02782 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-06-05

Order DetailsPrice
M02782

100μL

$370
M02782-30ul

30μL sample

$99
M02782-carrier-free-1ml

Carrier Free

$2590

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
M02782
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.