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Product Info Summary
| SKU: | M02782 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody
View all LPAR1/LPA1/EDG-2 Antibodies
SKU/Catalog Number
M02782
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody catalog # M02782. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02782)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACGE-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EDG2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02782 is reactive to LPAR1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa, 90 kDa
Calculated molecular weight
98399 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02782 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of EDG2 expression in A375 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For LPAR1 (Source: Uniprot.org, NCBI)
Gene Name
LPAR1
Full Name
Lysophosphatidic acid receptor 1
Weight
98399 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Cytoplasmic aconitate hydratase;Aconitase;4.2.1.3;Citrate hydro-lyase;Ferritin repressor protein;Iron regulatory protein 1;IRP1;Iron-responsive element-binding protein 1;IRE-BP 1;ACO1;IREB1; LPAR1 EDG2, Gpcr26, LPA1, Mrec1.3, VZG1, edg-2, rec.1.3, vzg-1 lysophosphatidic acid receptor 1 lysophosphatidic acid receptor 1|LPA receptor 1|LPA-1|endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2|lysophosphatidic acid receptor Edg-2|ventricular zone gene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LPAR1, check out the LPAR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LPAR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (M02782)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody
Question
Is M02782 a recombinant antibody? What is the expression system and the transfection method?
Verified customer
Asked: 2021-01-12
Answer
The Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody (M02782) is a recombinant antibody. Expression system is plasmid transfection with 293F without using any viral particles.
Boster Scientific Support
Answered: 2021-01-12
Question
What sample target on Western Blot application was tested with M02782?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of EDG2 expression in A375 cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
I see that the anti-EDG2 Monoclonal antibody M02782 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-EDG2 Monoclonal antibody M02782. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-19
Question
Will M02782 anti-EDG2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
As indicated on the product datasheet, M02782 anti-EDG2 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-EDG2 Monoclonal antibody M02782 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
J. Jha
Verified customer
Asked: 2016-12-13
Answer
The anti-EDG2 Monoclonal antibody (M02782) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-13
Question
Does anti-EDG2 Monoclonal antibody M02782 work for IP with liver?
Z. Krishna
Verified customer
Asked: 2014-10-08
Answer
According to the expression profile of liver, LPAR1 is highly expressed in liver. So, it is likely that anti-EDG2 Monoclonal antibody M02782 will work for IP with liver.
Boster Scientific Support
Answered: 2014-10-08
Question
I was wanting to use your anti-EDG2 Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Z. Johnson
Verified customer
Asked: 2013-07-31
Answer
As indicated on the product datasheet, M02782 anti-EDG2 Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-31
Question
Does anti-EDG2 Monoclonal antibody M02782 work on monkey WB with liver?
N. Johnson
Verified customer
Asked: 2013-06-05
Answer
Our lab technicians have not validated anti-EDG2 Monoclonal antibody M02782 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EDG2 Monoclonal antibody M02782 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-05
