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Product Info Summary
| SKU: | A03805-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-HIF3/HIF3A Antibody Picoband®
View all HIF-3 alpha Antibodies
SKU/Catalog Number
A03805-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HIF3/HIF3A Antibody Picoband® catalog # A03805-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF3/HIF3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03805-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HIF3/HIF3A recombinant protein (Position: Q29-Q480).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03805-3 is reactive to HIF3A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
64133 MW
Background of HIF-3 alpha
Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03805-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human A431 whole cell, human Caco-2 whole cell, human U-87MG whole cell, human U2OS whole cell, human K562 whole cell, human PC-3 whole cell, rat lung tissue, rat skeletal muscle tissue, mouse lung tissue, mouse NIH3T3 whole cell
FCM: U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Click image to see more details
Figure 2. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Click image to see more details
Figure 3. Flow Cytometry analysis of U87 cells using anti-HIF3A antibody (A03805-3).
Overlay histogram showing U87 cells stained with A03805-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF3A Antibody (A03805-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For HIF3A (Source: Uniprot.org, NCBI)
Gene Name
HIF3A
Full Name
Hypoxia-inducible factor 3-alpha
Weight
64133 MW
Alternative Names
"Hypoxia-inducible factor 3-alpha; HIF-3-alpha; HIF3-alpha; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7; HIF3A; BHLHE17, MOP7, PASD7 Hif3a|Ipas, MO, MOP7, N, NEPAS, bHLHe1, bHLHe17|hypoxia inducible factor 3, alpha subunit|hypoxia-inducible factor 3-alpha|HIF-3-alpha|HIF3-alpha|HIF3-alpha-1|basic-helix-loop-helix-PAS protein MOP7|hypoxia inducible factor three alpha|inhibitory PAS domain protein|member of PAS protein 7|neonatal and embryonic PAS protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIF3A, check out the HIF3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIF3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF3/HIF3A Antibody Picoband® (A03805-3)
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Customer Q&As
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2 Customer Q&As for Anti-HIF3/HIF3A Antibody Picoband®
Question
I hope that you are well. Could you please advise the Molecular weight of the western blot?
Verified customer
Asked: 2022-07-07
Answer
Hello, thank you for your question. The detected molecular weight of the western blot is around 72KD. Please see the result images on the product page. https://www.bosterbio.com/anti-hif3-hif3a-picoband-trade-antibody-a03805-3-boster.html
Boster Scientific Support
Answered: 2022-07-08
Question
What is the detected Molecular weight for A03805-3 in western blot?
Verified customer
Asked: 2022-06-22
Answer
The detected molecular weight of the western blot is around 72KD. Please see the result images on the product page of the Anti-HIF3/HIF3A Antibody Picoband™ (A03805-3). https://www.bosterbio.com/anti-hif3-hif3a-picoband-trade-antibody-a03805-3-boster.html
Boster Scientific Support
Answered: 2022-06-23
