Anti-Phospholipase A2/PLA2G4A Antibody Picoband®

Product Name

Anti-Phospholipase A2/PLA2G4A Antibody Picoband®

View all PLA2G4A Antibodies

SKU/Catalog Number

PA2047

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Phospholipase A2/PLA2G4A Antibody catalog # PA2047. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospholipase A2/PLA2G4A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2047)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2047 is reactive to PLA2G4A in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

58 kDa

Calculated molecular weight

85239 MW

Background of PLA2G4A

PLA2G4A (Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al. (1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al. (1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue (s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands (Skorecki, 1995).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2047 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Testis Tissue, Rat Brain Tissue, A549 Cell, COLO320 Cell,

Validation Images & Assay Conditions

Gene/Protein Information For PLA2G4A (Source: Uniprot.org, NCBI)

Alternative Names

Cytosolic phospholipase A2;cPLA2;Phospholipase A2 group IVA;Phospholipase A2;3.1.1.4;Phosphatidylcholine 2-acylhydrolase;Lysophospholipase;3.1.1.5;PLA2G4A;CPLA2, PLA2G4; PLA2G4A GURDP, PLA2G4, cPLA2, cPLA2-alpha phospholipase A2 group IVA cytosolic phospholipase A2|calcium-dependent phospholipid-binding protein|lysophospholipase|phosphatidylcholine 2-acylhydrolase|phospholipase A2, group IVA (cytosolic, calcium-dependent)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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