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Product Info Summary
| SKU: | EK0902 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Rat MCP-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0902
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat MCP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat CCL2 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat MCP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0902)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q24-N148
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0902 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0902 is reactive to CCL2 in Rat samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0902 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL2
Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0902-CAP | Anti-Rat CCL2 Pre-coated 96-well strip microplate | 1 |
| EK0902-ST | Rat CCL2 Standard | 2 vials, 10 ng/tube |
| EK0902-DA | Rat CCL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.002 | 0.024 | 0.058 | 0.137 | 0.266 | 0.491 | 1.137 | 1.885 |
Data Example Images
Click image to see more details
Rat MCP-1/CCL2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL2, Dilution ratio of 1:5, concentration in serum is 300-900 pg/ml..
Some articles we found to cite concentrations of CCL2 in samples: 25511499 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat MCP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 41 | 257 | 473 | 40 | 251 | 504 |
| Standard deviation | 2.29 | 21.58 | 27.43 | 2.56 | 21.58 | 29.23 |
| CV (%) | 7.2% | 7.2% | 5.8% | 6.4% | 8.6% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 41 | 37 | 39 | 42 | 39 | 1.92 | 4.9% |
| Sample 2 | 257 | 231 | 216 | 228 | 233 | 14.94 | 6.4% |
| Sample 3 | 473 | 462 | 446 | 461 | 460 | 9.6 | 2% |
Protein Target Info & Infographic
Gene/Protein Information For CCL2 (Source: Uniprot.Org, NCBI)
Gene Name
CCL2
Full Name
C-C motif chemokine 2
Weight
16460 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 2;Immediate-early serum-responsive protein JE;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Small-inducible cytokine A2;CCL2;Je, Mcp1, Scya2; CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL2, check out the CCL2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat MCP-1 ELISA Kit PicoKine® (EK0902)
Hello CJ!
EK0902 has been cited in 43 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comparison of biomarkers in rat renal ischemia-reperfusion injury
Tanshinone IIA attenuates cerebral aneurysm formation by inhibiting the NF‑κB‑mediated inflammatory response
Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE−/− Mice through the ROS/MAPK/NF-κB Pathway
The Potassium SK Channel Activator NS309 Protects Against Experimental Traumatic Brain Injury Through Anti-Inflammatory and Immunomodulatory Mechanisms
Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer
Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
IL-17A promotes the neuroinflammation and cognitive function in sevoflurane anesthetized aged rats via activation of NF-κB signaling pathway
A20-Binding Inhibitor of NF-κB 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats
Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Rat MCP-1 ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for CCL2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-16
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-11-16
Question
Does the lyophilized Rat Ccl2 standard supplied in EK0902 contain Sodium Azide (in buffer composition before lyophilization)?
Verified customer
Asked: 2020-09-08
Answer
The lyophilized standard supplied in the Rat MCP-1 ELISA Kit PicoKine™ (EK0902) contains 0.38% of Sodium Azide, which is higher than in the buffer before lyophilization.
Boster Scientific Support
Answered: 2020-09-10
Question
How much tissue lysate sample does this product Rat MCP-1 ELISA Kit PicoKine require per test?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The Rat MCP-1 ELISA Kit PicoKine requires 100ul of sample in each well.
Boster Scientific Support
Answered: 2019-12-11
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CCL2 in Rat serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-08-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
C. Turner
Verified customer
Asked: 2019-05-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-05-28
Question
Q: Can CCL2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-10-16
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CCL2 using the EK0902 Rat CCL2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-03
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-02-03
Question
Q: how to analyze ELISA data? I measured CCL2 level in serum.
C. Lee
Verified customer
Asked: 2015-05-07
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-05-07
Question
I am interested to purchase the product Rat MCP-1 ELISA Kit PicoKine and would like to ask if this kit is suitable for detecting MCP-1 in rat urine?
Verified Customer
Verified customer
Asked: 2013-07-04
Answer
The product Rat MCP-1 ELISA Kit PicoKine has not been validated in rat urine samples however, in our previous testing with our ELISA kit it is likely to work with urine. We recommend some general guidelines for the preparation of urine samples; Collect the samples without adding stabilizers, spin down hard at 10000 x g for 1min, the aliquot freeze quickly in dry ice or methanol bath and store in -80C until use. We suggest to start with minimal dilution such as 2 fold since cytokine levels is often very low in urine.
Boster Scientific Support
Answered: 2013-07-04
