Click image to see more details
Product Info Summary
| SKU: | A00056-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CCL2 Antibody Picoband®
SKU/Catalog Number
A00056-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCL2 Antibody Picoband® catalog # A00056-4. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00056-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat CCL2 recombinant protein (Position: Q24-R96).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00056-4 is reactive to Ccl2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
25283 MW
Background of CCL2
Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00056-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: rat pancreas tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
Protein Target Info & Infographic
Gene/Protein Information For Ccl2 (Source: Uniprot.org, NCBI)
Gene Name
Ccl2
Full Name
C-C motif chemokine 2
Weight
25283 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 2; Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2; Ccl2; Je; Mcp1; Scya2 CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccl2, check out the Ccl2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccl2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCL2 Antibody Picoband® (A00056-4)
Hello CJ!
A00056-4 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Tff3 Deficiency and ER Stress in the Liver
Fluoxetine inhibited extracellular matrix of pulmonary artery and inflammation of lungs in monocrotaline-treated rats
The anti-inflammation effect of Moutan Cortex on advanced glycation end products-induced rat mesangial cells dysfunction and High-glucose–fat diet and streptozotocin-induced diabetic nephropathy rats
Salusin-%u03B2 not salusin-%u03B1 promotes vascular inflammation in ApoE-deficient mice via the I-%u03BAB%u03B1/NF-%u03BAB pathway
Notch2/Hes-1 pathway plays an important role in renal ischemia and reperfusion injury-associated inflammation and apoptosis and the %u03B3-secretase inhibitor DAPT has %u2026
Comparison of biomarkers in rat renal ischemia-reperfusion injury
Salusin-?, but Not Salusin-?, Promotes Human Umbilical Vein Endothelial Cell Inflammation via the p38 MAPK/JNK-NF-?B Pathway
Bindarit reduces the incidence of acute aortic dissection complicated lung injury via modulating NF-?B pathway
Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm
Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats
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Customer Reviews
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Customer Q&As
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19 Customer Q&As for Anti-CCL2 Antibody Picoband®
Question
I would like to know if this antibody Anti-CCL2 Antibody Picoband can be used for neutralization even though it contains azide.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We recommend for this antibody Anti-CCL2 Antibody Picoband that the azide be dialyzed out of the antibody preparation before any in vivo or in vitro studies.
Boster Scientific Support
Answered: 2020-02-04
Question
Our lab want to know about to test anti-CCL2 antibody A00056-4 on mouse urinary bladder for research purposes, then I may be interested in using anti-CCL2 antibody A00056-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The products we sell, including anti-CCL2 antibody A00056-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use your anti-CCL2 antibody for WB for mouse urinary bladder on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse urinary bladder identification?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
As indicated on the product datasheet, A00056-4 anti-CCL2 antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse urinary bladder in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Will A00056-4 anti-CCL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
You can see on the product datasheet, A00056-4 anti-CCL2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-04
Question
Does anti-CCL2 antibody A00056-4 work on feline WB with urinary bladder?
R. Parker
Verified customer
Asked: 2019-08-14
Answer
Our lab technicians have not validated anti-CCL2 antibody A00056-4 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CCL2 antibody A00056-4 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline urinary bladder in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
Is there a BSA free version of anti-CCL2 antibody A00056-4 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CCL2 antibody A00056-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
Question
I am looking for using your anti-CCL2 antibody for inflammatory response studies. Has this antibody been tested with western blotting on rat pancreas tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
I appreciate your inquiry. This A00056-4 anti-CCL2 antibody is tested on rat pancreas tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-22
Question
Is a blocking peptide available for product anti-CCL2 antibody (A00056-4)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We do provide the blocking peptide for product anti-CCL2 antibody (A00056-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-11
Question
See attached the WB image, lot number and protocol we used for urinary bladder using anti-CCL2 antibody A00056-4. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-20
Question
We were well pleased with the WB result of your anti-CCL2 antibody. However we have observed positive staining in glial tumor secreted using this antibody. Is that expected? Could you tell me where is CCL2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
From what I have seen in literature, glial tumor does express CCL2. Generally CCL2 expresses in secreted. Regarding which tissues have CCL2 expression, here are a few articles citing expression in various tissues:
Glial tumor, Pubmed ID: 2465924
Pancreas, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-20
Question
We purchased anti-CCL2 antibody for ELISA on urinary bladder last year. I am using mouse, and We are going to use the antibody for WB next. My question regards examining urinary bladder as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
I viewed the website and datasheets of our anti-CCL2 antibody and it seems that A00056-4 has been validated on mouse in both ELISA and WB. Thus A00056-4 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-17
Question
Will anti-CCL2 antibody A00056-4 work for WB with urinary bladder?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
According to the expression profile of urinary bladder, CCL2 is highly expressed in urinary bladder. So, it is likely that anti-CCL2 antibody A00056-4 will work for WB with urinary bladder.
Boster Scientific Support
Answered: 2019-03-05
Question
We have observed staining in mouse pancreas. Are there any suggestions? Is anti-CCL2 antibody supposed to stain pancreas positively?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
From literature pancreas does express CCL2. From Uniprot.org, CCL2 is expressed in smooth muscle tissue, glial tumor, urinary bladder, pancreas, among other tissues. Regarding which tissues have CCL2 expression, here are a few articles citing expression in various tissues:
Glial tumor, Pubmed ID: 2465924
Pancreas, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-02-21
Question
I see that the anti-CCL2 antibody A00056-4 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-29
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder using anti-CCL2 antibody A00056-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-30
Question
My question regarding product A00056-4, anti-CCL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00056-4 anti-CCL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-08
Question
I want to purchase Anti-CCL2 Antibody Picoband for normal and Tg mouse brain cortex by Western Blot. How much total protein you load to pick up the MCP-1 using this antibody at the recommended 1/2000 dilution? Would this antibody also pick up the protein from mouse brain cortex?
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
The product Anti-CCL2 Antibody Picoband has been tested by WB using RAW cells treated with LPS for 15 hours. 10ug of lysate and 10ug of supernatant was loaded into the gel and a noticeable band of 25 kDa was detected.
Boster Scientific Support
Answered: 2017-05-16
Question
Is this A00056-4 anti-CCL2 antibody reactive to the isotypes of CCL2?
C. Mangal
Verified customer
Asked: 2015-08-10
Answer
The immunogen of A00056-4 anti-CCL2 antibody is E. coli-derived rat CCL2 recombinant protein (Position: Q24-R96). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-08-10
Question
We are currently using anti-CCL2 antibody A00056-4 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
B. Walker
Verified customer
Asked: 2015-07-02
Answer
The anti-CCL2 antibody (A00056-4) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-02
