Click image to see more details
Product Info Summary
| SKU: | A00854-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PLA2G4A Antibody (Center)
SKU/Catalog Number
A00854-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-PLA2G4A Antibody (Center) (Catalog # A00854-1). Tested in WB, Flow Cytometry, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PLA2G4A Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00854-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the Central region of human PLA2G4A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00854-1 is reactive to PLA2G4A in Human
Reconstitution
Calculated molecular weight
85239 Da
Background of PLA2G4A
PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00854-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Confocal immunofluorescent analysis of PLA2G4A Antibody (Center) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Click image to see more details
Western blot analysis of lysate from 293 cell line, using PLA2G4A Antibody (Center) . A00854-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Click image to see more details
Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Click image to see more details
Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For PLA2G4A (Source: Uniprot.org, NCBI)
Gene Name
PLA2G4A
Full Name
Cytosolic phospholipase A2
Weight
85239 Da
Alternative Names
Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4A, CPLA2, PLA2G4 PLA2G4A GURDP, PLA2G4, cPLA2, cPLA2-alpha phospholipase A2 group IVA cytosolic phospholipase A2|calcium-dependent phospholipid-binding protein|lysophospholipase|phosphatidylcholine 2-acylhydrolase|phospholipase A2, group IVA (cytosolic, calcium-dependent)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLA2G4A, check out the PLA2G4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLA2G4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLA2G4A Antibody (Center) (A00854-1)
Hello CJ!
No publications found for A00854-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PLA2G4A Antibody (Center)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PLA2G4A Antibody (Center)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
