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Product Info Summary
| SKU: | A01384 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PKR(Ab-446) EIF2AK2 Antibody
SKU/Catalog Number
A01384
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PKR(Ab-446) EIF2AK2 Antibody (Catalog # A01384). Tested in WB, IHC, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKR(Ab-446) EIF2AK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01384)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.444~448 (K-R-T-R-S) derived from Human PKR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01384 is reactive to EIF2AK2 in Human, Rat
Reconstitution
Calculated molecular weight
62094 MW
Background of PKR
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Abujiang Pataer, et,al. (2002) Cancer Res ; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol ; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem ; 281: 18734 - 18745.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01384 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR(Ab-446) antibody A01384(left) or the same antibody preincubated with blocking peptide(right).
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Western blot analysis of extracts from 293, Hela and C6 cells using PKR(Ab-446) antibody A01384.
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Immunofluorescence staining of methanol-fixed Hela cells using PKR(Ab-446) antibody A01384.
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK2 (Source: Uniprot.org, NCBI)
Gene Name
EIF2AK2
Full Name
Interferon-induced, double-stranded RNA-activated protein kinase
Weight
62094 MW
Superfamily
protein kinase superfamily
Alternative Names
Interferon-induced, double-stranded RNA-activated protein kinase;2.7.11.1;Eukaryotic translation initiation factor 2-alpha kinase 2;eIF-2A protein kinase 2;Interferon-inducible RNA-dependent protein kinase;P1/eIF-2A protein kinase;Protein kinase RNA-activated;PKR;Protein kinase R ;Tyrosine-protein kinase EIF2AK2;2.7.10.2;p68 kinase;EIF2AK2;PKR, PRKR; EIF2AK2 EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR eukaryotic translation initiation factor 2 alpha kinase 2 interferon-induced, double-stranded RNA-activated protein kinase|P1/eIF-2A protein kinase|double stranded RNA activated protein kinase|eIF-2A protein kinase 2|interferon-inducible elF2alpha kinase|p68 kinase|protein kinase R|protein kinase, interferon-inducible double stranded RNA dependent|protein phosphatase 1, regulatory subunit 83|tyrosine-protein kinase EIF2AK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2AK2, check out the EIF2AK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2AK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKR(Ab-446) EIF2AK2 Antibody (A01384)
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