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Product Info Summary
| SKU: | A00653T197 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PKA Alpha / Beta cat (phospho-T197) PRKACA Antibody
SKU/Catalog Number
A00653T197
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PKA Alpha / Beta cat (phospho-T197) PRKACA Antibody catalog # A00653T197. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKA Alpha / Beta cat (phospho-T197) PRKACA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00653T197)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human PKAα around the phosphorylation site of Threonine 197.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00653T197 is reactive to PRKACA in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
40590 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00653T197 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of PRKACA using Anti-PKA Alpha / Beta cat (phospho-T197) PRKACA Antibody (A00653T197).
Immunohistochemistry (IHC) analyzes of p-PKAα/β cat (T197) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-PKA Alpha / Beta cat (phospho-T197) PRKACA Antibody A00653T197
Western blot (WB) analysis of P-PKAα/β cat (T197) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:The Testis tissue lysate of Mouse(40ug)
Lane4:The Testis tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PRKACA (Source: Uniprot.org, NCBI)
Gene Name
PRKACA
Full Name
cAMP-dependent protein kinase catalytic subunit alpha
Weight
40590 MW
Superfamily
protein kinase superfamily
Alternative Names
cAMP-dependent protein kinase catalytic subunit alpha;PKA C-alpha;2.7.11.11;PRKACA;PKACA; PRKACA CAFD1, PKACA, PPNAD4 protein kinase cAMP-activated catalytic subunit alpha cAMP-dependent protein kinase catalytic subunit alpha|PKA C-alpha|protein kinase A catalytic subunit|protein kinase, cAMP-dependent, alpha catalytic subunit|protein kinase, cAMP-dependent, catalytic, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKACA, check out the PRKACA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKACA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKA Alpha / Beta cat (phospho-T197) PRKACA Antibody (A00653T197)
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