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Product Info Summary
| SKU: | A00653 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody
SKU/Catalog Number
A00653
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody catalog # A00653. Tested in ELISA, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00653)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00653 is reactive to PRKACA in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
40590 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00653 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of COLO205 cells using PKAalpha/beta/gamma cat Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using PKAalpha/beta/gamma cat Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PRKACA (Source: Uniprot.org, NCBI)
Gene Name
PRKACA
Full Name
cAMP-dependent protein kinase catalytic subunit alpha
Weight
40590 MW
Superfamily
protein kinase superfamily
Alternative Names
cAMP-dependent protein kinase catalytic subunit alpha;PKA C-alpha;2.7.11.11;PRKACA;PKACA; PRKACA CAFD1, PKACA, PPNAD4 protein kinase cAMP-activated catalytic subunit alpha cAMP-dependent protein kinase catalytic subunit alpha|PKA C-alpha|protein kinase A catalytic subunit|protein kinase, cAMP-dependent, alpha catalytic subunit|protein kinase, cAMP-dependent, catalytic, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKACA, check out the PRKACA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKACA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody (A00653)
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No publications found for A00653
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody
Question
I see that the anti-PKA alpha/ beta/ gamma cat antibody A00653 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-24
Question
Is a blocking peptide available for product anti-PKA alpha/ beta/ gamma cat antibody (A00653)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We do provide the blocking peptide for product anti-PKA alpha/ beta/ gamma cat antibody (A00653). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-17
Question
We want to test anti-PKA alpha/ beta/ gamma cat antibody A00653 on rat testis for research purposes, then I may be interested in using anti-PKA alpha/ beta/ gamma cat antibody A00653 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The products we sell, including anti-PKA alpha/ beta/ gamma cat antibody A00653, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-PKA alpha/ beta/ gamma cat antibody A00653 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
The anti-PKA alpha/ beta/ gamma cat antibody (A00653) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-06
Question
Would A00653 anti-PKA alpha/ beta/ gamma cat antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
It shows on the product datasheet, A00653 anti-PKA alpha/ beta/ gamma cat antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-15
Question
I was wanting to use your anti-PKA alpha/ beta/ gamma cat antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
You can see on the product datasheet, A00653 anti-PKA alpha/ beta/ gamma cat antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-27
Question
See below the WB image, lot number and protocol we used for testis using anti-PKA alpha/ beta/ gamma cat antibody A00653. Please let me know if you require anything else.
L. Baker
Verified customer
Asked: 2016-01-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-13
