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Product Info Summary
| SKU: | M05307-7 |
|---|---|
| Size: | 50ul |
| Reactive Species: | Bovine, Canine, Equine, Human, Mouse, Pig, Rat |
| Host: | Chicken |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Phosphorylated Neurofilament NF-H NEFH Antibody
SKU/Catalog Number
M05307-7
Size
50ul
Description
Boster Bio Anti-Phosphorylated Neurofilament NF-H NEFH Antibody catalog # M05307-7. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Canine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phosphorylated Neurofilament NF-H NEFH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05307-7)
Host
Chicken
Contents
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Native NF-H purified from bovine spinal cord.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05307-7 is reactive to NEFH in Bovine, Canine, Equine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
112479 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05307-7 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000,000. IF/ICC and IHC: 1:100,000. ELISA-capture antibody
Validation Images & Assay Conditions
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Western blot analysis of spinal cord lysates from different species using chicken pAb to NF-H, M05307-7, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H (not shown, but see reference 1).
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Immunohistological analysis of a rat cerebellum section stained with chicken pAb to NF-H, M05307-7, dilution 1:5,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.
Protein Target Info & Infographic
Gene/Protein Information For NEFH (Source: Uniprot.org, NCBI)
Gene Name
NEFH
Full Name
Neurofilament heavy polypeptide
Weight
112479 MW
Superfamily
intermediate filament family
Alternative Names
Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;NEFH;KIAA0845, NFH; NEFH CMT2CC, NFH neurofilament heavy neurofilament heavy polypeptide|200 kDa neurofilament protein|NF-H|neurofilament triplet H protein|neurofilament, heavy polypeptide 200kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEFH, check out the NEFH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEFH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phosphorylated Neurofilament NF-H NEFH Antibody (M05307-7)
Hello CJ!
M05307-7 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead
Species: Human
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