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Product Info Summary
| SKU: | A02638-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-PIP5K PIKFYVE Antibody
View all PIKFyve/PIP5K3 Antibodies
SKU/Catalog Number
A02638-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PIP5K PIKFYVE Antibody (Catalog # A02638-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIP5K PIKFYVE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02638-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PIP5K.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02638-1 is reactive to PIKFYVE in Human, Mouse
Reconstitution
Calculated molecular weight
237136 MW
Background of PIKFyve/PIP5K3
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.
Ota T., Nat. Genet. 36:40-45(2004).
Sbrissa D., J. Biol. Chem. 277:6073-6079(2002).
Brill L.M., Anal. Chem. 76:2763-2772(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02638-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, using PIP5K antibody A02638-1.
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Immunofluorescence analysis of COS7 cells, using PIP5K antibody A02638-1.
Protein Target Info & Infographic
Gene/Protein Information For PIKFYVE (Source: Uniprot.org, NCBI)
Gene Name
PIKFYVE
Full Name
1-phosphatidylinositol 3-phosphate 5-kinase
Weight
237136 MW
Alternative Names
1-phosphatidylinositol 3-phosphate 5-kinase;Phosphatidylinositol 3-phosphate 5-kinase;2.7.1.150;FYVE finger-containing phosphoinositide kinase;PIKfyve;Phosphatidylinositol 3-phosphate 5-kinase type III;PIPkin-III;Type III PIP kinase;PIKFYVE;KIAA0981, PIP5K3; Pikfyve|5230400C17Rik, P, Pip, Pip5k, Pip5k3, Pipk5k3, PipkIII, p235|phosphoinositide kinase, FYVE type zinc finger containing|1-phosphatidylinositol 3-phosphate 5-kinase|FYVE finger-containing phosphoinositide kinase|PIPkin-III|PtdIns(4)P-5-kinase|phosphatidylinositol 3-phosphate 5-kinase type III|phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III|phosphatidylinositol-4-phosphate 5-kinase, type III|phosphoinositide kinase, FYVE finger containing|serine-protein kinase PIKFYVE|type III PIP kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIKFYVE, check out the PIKFYVE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIKFYVE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIP5K PIKFYVE Antibody (A02638-1)
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