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Product Info Summary
| SKU: | A07445 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-WSB1 Antibody
SKU/Catalog Number
A07445
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WSB1 Antibody (Catalog # A07445). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
WSB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-WSB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07445)
Host
Rabbit
Contents
WSB1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1. The immunogen is located within amino acids 70 - 120 of WSB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
WSB1 antibody is human specific.
Reactive Species
A07445 is reactive to WSB1 in Human
Applications
A07445 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
47432 MW
Background of WSB1
The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1), is a member of the WD-protein subfamily (1). It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1,2). WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3). Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WSB1 in human colon tissue lysate with WSB1 antibody at 1 μg/ml.
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Immunohistochemistry of WSB1 in human colon tissue with WSB1 antibody at 5 μg/mL.
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Immunofluorescence of WSB1 in human colon tissue with WSB1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For WSB1 (Source: Uniprot.org, NCBI)
Gene Name
WSB1
Full Name
WD repeat and SOCS box-containing protein 1
Weight
47432 MW
Alternative Names
DKFZp564B0482; SOCS box-containing WD protein SWiP-1; SWIP1DKFZp564A122; WD repeat and SOCS box containing 1; WD repeat and SOCS box-containing 1; WD repeat and SOCS box-containing protein 1; WSB-1 WSB1 SWIP1, WSB-1 WD repeat and SOCS box containing 1 WD repeat and SOCS box-containing protein 1|SOCS box-containing WD protein SWiP-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WSB1, check out the WSB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WSB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WSB1 Antibody (A07445)
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