Product Info Summary
SKU: | P02638 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-Phospho PIP5K (Ser307) Antibody
View all PIKFyve/PIP5K3 Antibodies
SKU/Catalog Number
P02638
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho PIP5K (Ser307) Antibody (Catalog# P02638). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho PIP5K (Ser307) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02638)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PIP5K around the phosphorylation site of Ser307. Immunogen sequence location: 273-322
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02638 is reactive to Pikfyve in Human, Mouse, Rat
Applications
P02638 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
237136 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
![PIKFYVE primary antibodies P02638 IHC testing PIKFYVE primary antibodies P02638 IHC testing](https://www.bosterbio.com/media/catalog/product/P/I/PIKFYVE-primary-antibodies-P02638-IHC-testing.jpg)
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using PIP5K (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
![PIKFYVE primary antibodies P02638 IF testing PIKFYVE primary antibodies P02638 IF testing](https://www.bosterbio.com/media/catalog/product/P/I/PIKFYVE-primary-antibodies-P02638-IF-testing.jpg)
Click image to see more details
Immunofluorescence analysis of HeLa cells, using PIP5K (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For Pikfyve (Source: Uniprot.org, NCBI)
Gene Name
Pikfyve
Full Name
1-phosphatidylinositol 3-phosphate 5-kinase
Weight
237136 MW
Alternative Names
1-phosphatidylinositol-3-phosphate 5-kinase; EC 2.7.1.150; FAB1; FLJ37746; FYVE finger-containing phosphoinositide kinase; KIAA0981CFD; MGC40423; p235; Phosphatidylinositol-3-phosphate 5-kinase type III; Phosphatidylinositol-3-phosphate 5-kinase; phosphoinositide kinase, FYVE finger containing; PIKFyve; PIP5K; PIP5K3; PIP5K3FAB1; PIPkin-III; Type III PIP kinase; type III Pikfyve|5230400C17Rik, P, Pip, Pip5k, Pip5k3, Pipk5k3, PipkIII, p235|phosphoinositide kinase, FYVE type zinc finger containing|1-phosphatidylinositol 3-phosphate 5-kinase|FYVE finger-containing phosphoinositide kinase|PIPkin-III|PtdIns(4)P-5-kinase|phosphatidylinositol 3-phosphate 5-kinase type III|phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III|phosphatidylinositol-4-phosphate 5-kinase, type III|phosphoinositide kinase, FYVE finger containing|serine-protein kinase PIKFYVE|type III PIP kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pikfyve, check out the Pikfyve Infographic
![Pikfyve infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pikfyve: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho PIP5K (Ser307) Antibody (P02638)
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No publications found for P02638
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Customer Q&As
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7 Customer Q&As for Anti-Phospho PIP5K (Ser307) Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Phospho PIP5K (Ser307) antibody P02638. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-03
Question
We are currently using anti-Phospho PIP5K (Ser307) antibody P02638 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The anti-Phospho PIP5K (Ser307) antibody (P02638) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-02
Question
Will anti-Phospho PIP5K (Ser307) antibody P02638 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of cervix carcinoma, PIKFYVE is highly expressed in cervix carcinoma. So, it is likely that anti-Phospho PIP5K (Ser307) antibody P02638 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2019-08-30
Question
I see that the anti-Phospho PIP5K (Ser307) antibody P02638 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-20
Question
Is a blocking peptide available for product anti-Phospho PIP5K (Ser307) antibody (P02638)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
We do provide the blocking peptide for product anti-Phospho PIP5K (Ser307) antibody (P02638). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-13
Question
Do you have a BSA free version of anti-Phospho PIP5K (Ser307) antibody P02638 available?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho PIP5K (Ser307) antibody P02638 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-19
Question
My lab would like to test anti-Phospho PIP5K (Ser307) antibody P02638 on rat cervix carcinoma for research purposes, then I may be interested in using anti-Phospho PIP5K (Ser307) antibody P02638 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Krishna
Verified customer
Asked: 2016-01-22
Answer
The products we sell, including anti-Phospho PIP5K (Ser307) antibody P02638, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-01-22