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Product Info Summary
| SKU: | A06618 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PIK3R4 Antibody
SKU/Catalog Number
A06618
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PIK3R4 Antibody (Catalog # A06618). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PIK3R4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-PIK3R4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06618)
Host
Rabbit
Contents
PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4. The immunogen is located within amino acids 1290 - 1340 of PIK3R4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PIK3R4 antibody is human, mouse and rat reactive.
Reactive Species
A06618 is reactive to PIK3R4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
153103 MW
Background of PIK3R4
PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06618 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 μg/ml.
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Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PIK3R4 (Source: Uniprot.org, NCBI)
Gene Name
PIK3R4
Full Name
Phosphoinositide 3-kinase regulatory subunit 4
Weight
153103 MW
Superfamily
protein kinase superfamily
Alternative Names
Phosphoinositide-3-kinase regulatory subunit 4, p150, Phosphoinositide 3-kinase adaptor protein, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, VPS15 PIK3R4 VPS15, p150 phosphoinositide-3-kinase regulatory subunit 4 phosphoinositide 3-kinase regulatory subunit 4|PI3-kinase p150 subunit|PI3-kinase regulatory subunit 4|phosphatidylinositol 3-kinase-associated p150|phosphoinositide 3-kinase adaptor protein|phosphoinositide-3-kinase, regulatory subunit 4, p150
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIK3R4, check out the PIK3R4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIK3R4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIK3R4 Antibody (A06618)
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