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Product Info Summary
| SKU: | A01651-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human SCRIBBLE DyLight® 550 conjugated Antibody
SKU/Catalog Number
A01651-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human SCRIBBLE DyLight® 550 conjugated Antibody catalog # A01651-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human SCRIBBLE DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01651-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01651-Dyl550 is reactive to SCRIB in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
174.885kDa
Background of Scribble
SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and epithelial polarization. Mechanistically, the human homolog is a scaffold protein linked to cellular differentiation centered on the regulation of epithelial as well as neuronal morphogenesis. Deficiency in SCRIB impairs many aspects of cell polarity and cell movement. SCRIB is also likely involved in establishing apical-basal polarity as well as progression from the G1 phase to S phase in the cell cycle as a result of its relationship with cell proliferation and exocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01651-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For SCRIB (Source: Uniprot.org, NCBI)
Gene Name
SCRIB
Full Name
Protein scribble homolog
Weight
174.885kDa
Superfamily
LAP (LRR and PDZ) protein family
Alternative Names
Protein scribble homolog; Scribble; hScrib; Protein LAP4; SCRIB; CRIB1; KIAA0147; LAP4; SCRB1; VARTUL SCRIB CRIB1, SCRB11, Vartul, SCRIB scribble planar cell polarity protein protein scribble homolog|scribbled homolog|scribbled planar cell polarity protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCRIB, check out the SCRIB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCRIB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human SCRIBBLE DyLight® 550 conjugated Antibody (A01651-Dyl550)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Human SCRIBBLE DyLight® 550 conjugated Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Human SCRIBBLE DyLight® 550 conjugated antibody A01651-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
Will A01651-Dyl550 anti-Human SCRIBBLE DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
As indicated on the product datasheet, A01651-Dyl550 anti-Human SCRIBBLE DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-17
Question
Is this A01651-Dyl550 anti-Human SCRIBBLE DyLight® 550 conjugated antibody reactive to the isotypes of SCRIB?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The immunogen of A01651-Dyl550 anti-Human SCRIBBLE DyLight® 550 conjugated antibody is E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-30
Question
We want to test anti-Human SCRIBBLE DyLight® 550 conjugated antibody A01651-Dyl550 on human lower esophagus mucosa for research purposes, then I may be interested in using anti-Human SCRIBBLE DyLight® 550 conjugated antibody A01651-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The products we sell, including anti-Human SCRIBBLE DyLight® 550 conjugated antibody A01651-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-Human SCRIBBLE DyLight® 550 conjugated antibody A01651-Dyl550 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-Human SCRIBBLE DyLight® 550 conjugated antibody (A01651-Dyl550) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
I see that the anti-Human SCRIBBLE DyLight® 550 conjugated antibody A01651-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-19
