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Product Info Summary
| SKU: | PA2215 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband®
SKU/Catalog Number
PA2215
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-E3 SUMO-protein ligase PIAS4 Antibody catalog # PA2215. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2215)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2215 is reactive to PIAS4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated molecular weight
56504 MW
Background of PIASy
E3 SUMO-protein ligase PIAS4, also known as protein inhibitor of activated STAT protein 4 (PIAS4) or protein inhibitor of activated STAT protein gamma (PIASg or PIASy) is an enzyme that in humans is encoded by the PIAS4 gene. This gene is mapped to 19p13.3. This gene plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. It functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This gene involved in gene silencing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2215 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Human Placenta Tissue, A431 Whole Cell
Validation Images & Assay Conditions
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Anti-PIAS4 antibody, PA2215, Western blotting
All lanes: Anti PIAS4 (PA2215) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: A431 Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
Protein Target Info & Infographic
Gene/Protein Information For PIAS4 (Source: Uniprot.org, NCBI)
Gene Name
PIAS4
Full Name
E3 SUMO-protein ligase PIAS4
Weight
56504 MW
Superfamily
PIAS family
Alternative Names
E3 SUMO-protein ligase PIAS4;6.3.2.-;PIASy;Protein inhibitor of activated STAT protein 4;Protein inhibitor of activated STAT protein gamma;PIAS-gamma;PIAS4;PIASG; PIAS4 PIAS-gamma, PIASY, Piasg, ZMIZ6 protein inhibitor of activated STAT 4 E3 SUMO-protein ligase PIAS4|RING-type E3 ubiquitin transferase PIAS4|protein inhibitor of activated STAT protein 4|protein inhibitor of activated STAT protein PIASy|protein inhibitor of activated STAT protein gamma|zinc finger, MIZ-type containing 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIAS4, check out the PIAS4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIAS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® (PA2215)
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No publications found for PA2215
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