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Product Info Summary
| SKU: | A02994 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody
SKU/Catalog Number
A02994
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody (Catalog # A02994). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PIAS4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02994)
Host
Rabbit
Contents
PIAS4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PIAS4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human PIAS4. The immunogen is located within amino acids 50 - 100 of PIAS4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of CITED2 are known to exist.
Reactive Species
A02994 is reactive to PIAS4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
56504 MW
Background of PIASy
The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS4. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS4 interacts with p53 and blocks its ability to induce Bax and p21 transcription. PIAS4 is also important in the control of the ubiquitin-dependent proteasomal degradation of the Ets-1 transcription factor. PIAS4 has been implicated in the DNA-damage response pathway and is thought to work in combination with PIAS1 for the productive association of 53BP1, BRCA1 and RNF168.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02994 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PIAS4 antibody can be used for detection of PIAS4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PIAS4 in HL60 cell lysate with PIAS4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of PIAS4 in rat brain tissue with PIAS4 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PIAS4 (Source: Uniprot.org, NCBI)
Gene Name
PIAS4
Full Name
E3 SUMO-protein ligase PIAS4
Weight
56504 MW
Superfamily
PIAS family
Alternative Names
PIASY, Piasg, ZMIZ6, PIASG, E3 SUMO-protein ligase PIAS4, PIASy, PIAS-gamma PIAS4 PIAS-gamma, PIASY, Piasg, ZMIZ6 protein inhibitor of activated STAT 4 E3 SUMO-protein ligase PIAS4|RING-type E3 ubiquitin transferase PIAS4|protein inhibitor of activated STAT protein 4|protein inhibitor of activated STAT protein PIASy|protein inhibitor of activated STAT protein gamma|zinc finger, MIZ-type containing 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIAS4, check out the PIAS4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIAS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E3 SUMO-protein ligase PIAS4 PIAS4 Antibody (A02994)
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