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Product Info Summary
| SKU: | RP1018 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CEACAM5 Antibody Picoband®
View all CEACAM5/CD66e Antibodies
SKU/Catalog Number
RP1018
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CEACAM5 Antibody catalog # RP1018. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEACAM5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1018)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Insect cells-derived human CEA recombinant protein (Position: full).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1018 is reactive to CEACAM5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120-200 kDa
Calculated molecular weight
76795 MW
Background of CEACAM5/CD66e
CEA (Carcinoembryonic antigen) is a glycoprotein involved in cell adhesion. It is normally produced during fetal development, but the production of CEA stops before birth. Therefore, it is not usually present in the blood of healthy adults, although levels are raised in heavy smokers. CEA is a glycosyl phosphatidyl inositol (GPI)-cell surface anchored glycoprotein whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1018 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human SW620 whole cell, human Caco-2 whole cell, mouse small intestine tissue, mouse stomach tissue, mouse lung tissue, mouse liver tissue, mouse NIH3T3 whole cell, mouse HEPA1-6 whole cell, mouse SP20 whole cell, rat RH35 whole cell
IHC: human colon caner tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CEA using anti-CEA antibody (RP1018).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: mouse small intestine tissue lysates,
Lane 4: mouse stomach tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse NIH3T3 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates,
Lane 9: mouse SP20 whole cell lysates,
Lane 10: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
Click image to see more details
Figure 2. IHC analysis of CEA using anti-CEA antibody (RP1018).
CEA was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CEA Antibody (RP1018) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CEACAM5 (Source: Uniprot.org, NCBI)
Gene Name
CEACAM5
Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Weight
76795 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA; CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic -related cell adhesion molecule 5|carcinoembryonic related cell adhesion molecule 5|meconium 100
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEACAM5, check out the CEACAM5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEACAM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEACAM5 Antibody Picoband® (RP1018)
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No publications found for RP1018
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Customer Q&As
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17 Customer Q&As for Anti-CEACAM5 Antibody Picoband®
Question
Does RP1018 anti-CEACAM5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
You can see on the product datasheet, RP1018 anti-CEACAM5 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-CEACAM5 antibody (RP1018)?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
We do provide the blocking peptide for product anti-CEACAM5 antibody (RP1018). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-17
Question
Is there a BSA free version of anti-CEACAM5 antibody RP1018 available?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CEACAM5 antibody RP1018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-03
Question
We have been able to see staining in human saliva. Any tips? Is anti-CEACAM5 antibody supposed to stain saliva positively?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
From literature saliva does express CEACAM5. From Uniprot.org, CEACAM5 is expressed in colonic mucosa, saliva, liver, among other tissues. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-10-11
Question
We were well pleased with the WB result of your anti-CEACAM5 antibody. However we have been able to see positive staining in colonic mucosa cell membrane using this antibody. Is that expected? Could you tell me where is CEACAM5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
According to literature, colonic mucosa does express CEACAM5. Generally CEACAM5 expresses in cell membrane. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-10-04
Question
Does anti-CEACAM5 antibody RP1018 work on pig ELISA with saliva?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Our lab technicians have not tested anti-CEACAM5 antibody RP1018 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CEACAM5 antibody RP1018 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig saliva in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-09
Question
Is this RP1018 anti-CEACAM5 antibody reactive to the isotypes of CEACAM5?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
The immunogen of RP1018 anti-CEACAM5 antibody is Insect cells-derived human CEA recombinant protein(Position: full). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-30
Question
We purchased anti-CEACAM5 antibody for WB on colonic mucosa a few months ago. I am using human, and We are going to use the antibody for IHC next. We need examining colonic mucosa as well as saliva in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
I have checked the website and datasheets of our anti-CEACAM5 antibody and I see that RP1018 has been tested on human in both WB and IHC. Thus RP1018 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-17
Question
We are currently using anti-CEACAM5 antibody RP1018 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
A. Mitchell
Verified customer
Asked: 2018-05-09
Answer
The anti-CEACAM5 antibody (RP1018) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-09
Question
I see that the anti-CEACAM5 antibody RP1018 works with IHC, what is the protocol used to produce the result images on the product page?
B. Rodriguez
Verified customer
Asked: 2018-01-08
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-08
Question
you antibody to test anti-CEACAM5 antibody RP1018 on human saliva for research purposes, then I may be interested in using anti-CEACAM5 antibody RP1018 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
The products we sell, including anti-CEACAM5 antibody RP1018, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-28
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for saliva using anti-CEACAM5 antibody RP1018. Let me know if you need anything else.
S. Patel
Verified customer
Asked: 2017-10-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-24
Question
See attached the WB image, lot number and protocol we used for saliva using anti-CEACAM5 antibody RP1018. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-17
Question
I was wanting to use your anti-CEACAM5 antibody for IHC for human saliva on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human saliva identification?
H. Krishna
Verified customer
Asked: 2017-08-08
Answer
As indicated on the product datasheet, RP1018 anti-CEACAM5 antibody has been tested for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human saliva in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-08
Question
Our lab want to know about using your anti-CEACAM5 antibody for apoptotic process studies. Has this antibody been tested with western blotting on liver tissue? We would like to see some validation images before ordering.
E. Edwards
Verified customer
Asked: 2016-07-06
Answer
We appreciate your inquiry. This RP1018 anti-CEACAM5 antibody is tested on human sw620 whole cell lysates, mouse lung tissue, small intestine tissue, stomach tissue, liver tissue, nih3t3 whole cell lysates, rat rh35 whole cell lysates. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-07-06
Question
I have a question about product RP1018, anti-CEACAM5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Li
Verified customer
Asked: 2015-12-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1018 anti-CEACAM5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-12-16
Question
Will anti-CEACAM5 antibody RP1018 work for IHC with saliva?
D. Krishna
Verified customer
Asked: 2014-09-08
Answer
According to the expression profile of saliva, CEACAM5 is highly expressed in saliva. So, it is likely that anti-CEACAM5 antibody RP1018 will work for IHC with saliva.
Boster Scientific Support
Answered: 2014-09-08
