Anti-CEACAM5 Antibody

Product Name

Anti-CEACAM5 Antibody

View all CEACAM5/CD66e Antibodies

SKU/Catalog Number

RP1018

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CEACAM5 Antibody catalog # RP1018. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CEACAM5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1018)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

Insect cells-derived human CEA recombinant protein (Position: full).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1018 is reactive to CEACAM5 in Human

Applications

RP1018 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

120-200 kDa

Calculated molecular weight

76795 MW

Background of CEACAM5/CD66e

CEA (Carcinoembryonic antigen) is a glycoprotein involved in cell adhesion. It is normally produced during fetal development, but the production of CEA stops before birth. Therefore, it is not usually present in the blood of healthy adults, although levels are raised in heavy smokers. CEA is a glycosyl phosphatidyl inositol (GPI)-cell surface anchored glycoprotein whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CEA using anti-CEA antibody (RP1018).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: mouse small intestine tissue lysates,
Lane 4: mouse stomach tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse NIH3T3 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates,
Lane 9: mouse SP20 whole cell lysates,
Lane 10: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.

Gene/Protein Information For CEACAM5 (Source: Uniprot.org, NCBI)

Alternative Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100 CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic antigen-related cell adhesion molecule 5|carcinoembryonic antigen related cell adhesion molecule 5|meconium antigen 100

For more info on CEACAM5, check out the CEACAM5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CEACAM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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