PIAS4 Antibodies

E3 SUMO-protein ligase PIAS4 (PIAS4)

Functions as an E3-type little ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a critical role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway.

Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168.

Gene Information

Human
Gene Name:	PIAS4
Uniprot:	Q8N2W9
Entrez:	51588

Family

Belongs to:
PIAS family

Alternative Names

E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; Piasg; PIAS-gamma; PIASY; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; zinc finger, MIZ-type containing 6; ZMIZ6

Molecular Weight

Mass (kDA):
56.504 kDA

Genomic Context

Human
Location:	19p13.3
Sequence:	19; NC_000019.10 (4007736..4039386)

Tissue Specificity

Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

Subcellular Localizations

Nucleus, PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Diseases

• Neoplasms
• Malignant Neoplasms
• Leukemia
• Carcinoma
• Malignant Neoplasm Of Breast
• Myeloid Leukemia
• Tumor Progression
• Cell Transformation, Neoplastic
• Tumor Angiogenesis
• Acute Promyelocytic Leukemia
• Mammary Neoplasms
• Dystrophy
• Inflammation

• Leukoencephalopathy, Progressive Multifocal
• Inflammatory Response
• Carcinogenesis
• Sarcotubular Myopathy
• Malignant Squamous Cell Neoplasm
• Muscular Dystrophies, Limb-girdle
• Muscular Dystrophy

Interacting Proteins

• ESRRA
• HIF1A
• HTT
• PARP1
• RELA

• SUMO2
• TADA3
• TP53

Pathways

• Conjugation
• Localization
• Cell Cycle
• Cell Proliferation
• Senescence
• Gene Silencing
• Immune Response
• Chromosome Segregation
• Anaphase
• Inflammatory Response
• Reverse Transcription
• Tissue Remodeling
• Cell Growth

• Angiogenesis
• Rna Interference
• Virion Assembly
• Cellular Homeostasis
• Cellular Senescence
• Regulation Of Signal Transduction
• Dna Integration

PTMs

• Sumoylation
• Phosphorylation
• Ubiquitination

• Reduction
• Cleavage
• Acetylation

• Dephosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PIAS4

Best Uses Of The PIAS4 Marker

The PIAS4 gene has been well-characterized in a variety human tissues and cell types. It is controlled by the presence of specific proteins in the body. This gene is responsible the production of neurotransmitters as well as cytokines. This gene is also associated with the development of a range of genetic disorders, including schizophrenia and epilepsy. This article will discuss the best uses of this gene marker and Steven Boster, who discovered them.

Enhanced Chemiluminescence-Detection (ECL).

ECL is a chemiluminescent method that uses antibodies conjugated to HP. The reaction results in excited intermediates that emit light at 450 nm wavelength from the enzyme and substrate. The enzyme-substrate reactions are the only ones that produce light. The signal output stops when the substrate has been exhausted.

ECL has many benefits over other methods, including multiple exposures that optimize signal-to noise ratios. It can detect very low protein concentrations while still allowing for high throughput. Chemiluminescence can also be used to quantify a wide range of biological materials. It is sensitive and highly accurate.

HRP is the most widely used enzyme for Western Blotting. Thermo scientific Pierce produces a variety of chemiluminescent substrates, each with varying levels sensitivity. The table below provides details about the characteristics of different substrate types. In addition to allowing for high sensitivity, HRP substrates have various chromogenic features, including fluorescence-activated red, blue, green, or yellow.

Amersham ECL Plus offers twice as much sensitivity, while Amersham ECL Prim allows for repeated exposures. Amersham ECL Prime can yield signal intensities up to five times greater that Amersham ECL Plus, making them easier to process multiple blots within a single experiment.

Colorimetric detection (DAB).

Colorimetric detection is a method of detecting cancer in patients by assessing the concentration of a specific marker on the sample. There are a number of advantages to this technique, including ease of use, cost-effectiveness, and minimal instrumentation. Because it can be performed using a smartphone, doctors have great potential to use it. Learn more about the benefits of this technique.

Colorimetric Western blotting is the simplest, most cost-effective, and easiest method. A secondary antibody that is conjugated to an enzyme reader reacts with a dye to produce a visual detectable signal. This technique identifies the protein of interest directly on the blotting membrane. The result is a band of color that can be viewed by the human eye.

The assay was used for the detection of bladder cancer. It was evaluated using six different labeled protein array assays. The detection antibodies used were TMB1 (trimethylene-B) and oxidized carbon NPs (oCNPs). In both methods, the signal was equally stable in both synthetic urine (1:3) and assay buffer. The results were comparable. However the assay for detecting PIAS4 in synthetic urine (1:3) was found to be more reliable and accurate.

This method offers many benefits, including increased sensitivity. You can detect concentrations of between 1 and 25 ng/mL HRP with a color-gradient. The measurement obtained from a stopped reaction is stable over several hours, whereas the signal from an unstopped reaction remains unstable over a period of time. Colorimetric detection is an effective method of cancer detection.

Membrane staining improves protein transfer efficiency

The Protein Blotting workflow involves blocking the membrane to prevent non-specific antibody binding and then transferring the target protein onto the blot. Next, antibodies and a probe (fluorophores, isotopes, or enzymes) are added onto the blot in order to locate the target protein on the membrane. Protein transfer can prove difficult. Many factors affect the efficiency, including the molecularweight and gel thickness of the target protein as well as the method used.

The proteins were incubated with NC and PVDF membranes. ECL-reagents were used for detection. After washing, membranes had to be re-probed by BSA and other antibodies. The PVDF membrane had a better reprobing capability than the NC membrane, especially when used with glycoproteins. The PVDF membrane was more able to bind the glycoprotein. In the three other cases, PVDF membranes had greater advantages.

To avoid bubbles, place the sample comb carefully on top of the membrane. Make sure there are no gaps between the teeth of the comb. For 30-60 minutes, incubation should take place at 37°C. To remove unbound antibody, the membrane should then be gently washed three more times with TBS Wash Buffer. The membrane should then be incubated with a secondary antibody in a 3:1 ratio.

The second step involved the preparation of the samples. The samples were electroblotted onto PVDF membranes and resolved with 10% SDS-PAGE. The PVDF membrane is sensitiver and produces less nonspecific staining. However, it can remove very small molecular substances when washed. Additionally, it is sensitive and fragile. The total band intensities were quantified using a Student t-test to compare the two fixation groups.

To analyze the efficiency in protein transfer by membrane staining was first achieved by separating proteins using 10% SDS–PAGE. The proteins were then transferred to either nitrocellulose or PVDF membranes. Membranes were then cut into eight pieces for the fixation treatment. The exposed time was identical to the traditional fixation method. Finally, the bands were visualized using ECL Plus reagents.

History of Steven Boster

With the help of public records, you can search Steve Boster's past online. This database contains Steve's current and past addresses, cell phone numbers, email addresses, and known relatives. You can also search Steve’s public files by age and state. You can also narrow your search using the "Steve Boster" filter to find someone who matches the criteria you are looking for. The most important piece of Steve Boster's history is his personal information.

Steve Boster was a Joliet, IL, native on November 13, 1952. He was a veteran of the U.S. Army, and was a Concordia Hall member in Staunton, VA. He is survived in death by four siblings, Sandra Blanton; Jack Boster; Tammy Boster. He also has many nieces & nephews.