Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody

Product Name

Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody

View all YAP1 Antibodies

SKU/Catalog Number

P00116

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody catalog # P00116. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00116)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GAB-25

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human YAP1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00116 is reactive to YAP1 in Human, Mouse, Rat

Applications

P00116 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

70, 75 kDa

Calculated molecular weight

54462 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:20000
IHC 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of YAP1 using anti-YAP1 antibody (P00116).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat RH35 whole cell lysates,
Lane 4: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified monoclonal antibody (Catalog # P00116) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 70, 75 kDa. The expected band size for YAP1 is at 54 kDa.

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Immunohistochemical analysis of paraffin-embedded human stomach, using Phospho-YAP1 (S127) Antibody.

Gene/Protein Information For YAP1 (Source: Uniprot.org, NCBI)

Alternative Names

65 kDa Yes-associated protein; YAP; YAP1; YAP2; YAP65; YAP65yes-associated protein 2; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein beta; yes-associated protein delta; YKI; Yorkie Homolog YAP1 COB1, YAP, YAP2, YAP65, YKI Yes1 associated transcriptional regulator transcriptional coactivator YAP1|65 kDa Yes-associated protein|Yes associated protein 1|protein yorkie homolog|yes-associated protein 2|yes-associated protein YAP65 homolog|yorkie homolog

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In this infographic, you will see the following information for YAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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