Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M00116 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-YAP1/Yap Rabbit Monoclonal Antibody
SKU/Catalog Number
M00116
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-YAP1/Yap Rabbit Monoclonal Antibody catalog # M00116. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-YAP1/Yap Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00116)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CIE-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human YAP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00116 is reactive to YAP1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
54462 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00116 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human 293T whole cell
IHC: human uterus tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of YAP1 using anti-YAP1 antibody (M00116).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified monoclonal antibody (Catalog # M00116) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 70 kDa. The expected band size for YAP1 is at 54 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human uterus, using YAP1 Antibody.
Click image to see more details
Immunofluorescent analysis of MCF7 cells, using YAP1 Antibody .
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For YAP1 (Source: Uniprot.org, NCBI)
Gene Name
YAP1
Full Name
Transcriptional coactivator YAP1
Weight
54462 MW
Superfamily
YAP1 family
Alternative Names
Transcriptional coactivator YAP1;Yes-associated protein 1;Protein yorkie homolog;Yes-associated protein YAP65 homolog;YAP1;YAP65; YAP1 COB1, YAP, YAP2, YAP65, YKI Yes1 associated transcriptional regulator transcriptional coactivator YAP1|65 kDa Yes-associated protein|Yes associated protein 1|protein yorkie homolog|yes-associated protein 2|yes-associated protein YAP65 homolog|yorkie homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YAP1, check out the YAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-YAP1/Yap Rabbit Monoclonal Antibody (M00116)
Hello CJ!
No publications found for M00116
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-YAP1/Yap Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-YAP1/Yap Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-YAP1/Yap Rabbit Monoclonal Antibody
Question
I see that the anti-YAP1/Yap Rabbit Monoclonal antibody M00116 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-05
Question
I was wanting to use your anti-YAP1/Yap Rabbit Monoclonal antibody for WB for human esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
As indicated on the product datasheet, M00116 anti-YAP1/Yap Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-12
Question
Can you help my question with product M00116, anti-YAP1/Yap Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Mitchell
Verified customer
Asked: 2018-05-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00116 anti-YAP1/Yap Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-10
Question
Is this M00116 anti-YAP1/Yap Rabbit Monoclonal antibody reactive to the isotypes of YAP1?
T. Brown
Verified customer
Asked: 2015-08-12
Answer
The immunogen of M00116 anti-YAP1/Yap Rabbit Monoclonal antibody is A synthesized peptide derived from human YAP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-08-12
Question
Would anti-YAP1/Yap Rabbit Monoclonal antibody M00116 work for WB with esophagus?
R. Moore
Verified customer
Asked: 2013-08-16
Answer
According to the expression profile of esophagus, YAP1 is highly expressed in esophagus. So, it is likely that anti-YAP1/Yap Rabbit Monoclonal antibody M00116 will work for WB with esophagus.
Boster Scientific Support
Answered: 2013-08-16
