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Product Info Summary
| SKU: | MA1085 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)
SKU/Catalog Number
MA1085
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) catalog # MA1085. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1085)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
PKB-175
Isotype
Mouse IgG1
Immunogen
Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1085 is reactive to Akt1 in Chicken, Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
55735 MW
Background of Akt1
PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1085 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken
Positive Control
WB: human MCF-7 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PKB alpha using anti-PKB alpha antibody (MA1085).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PKB alpha antigen affinity purified monoclonal antibody (Catalog # MA1085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PKB alpha at approximately 60 kDa. The expected band size for PKB alpha is at 56 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Akt1 (Source: Uniprot.org, NCBI)
Gene Name
Akt1
Full Name
RAC-alpha serine/threonine-protein kinase
Weight
55735 MW
Superfamily
protein kinase superfamily
Alternative Names
RAC-alpha serine/threonine-protein kinase;2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;RAC-PK-alpha;Akt1; AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Akt1, check out the Akt1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Akt1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) (MA1085)
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Customer Q&As
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16 Customer Q&As for Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)
Question
My lab would like using your anti-PKB Alpha antibody (Monoclonal, PKB-175) for lipopolysaccharide-mediated signaling pathway studies. Has this antibody been tested with western blotting on rat ovary tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We appreciate your inquiry. This MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) is tested on rat ovary tissue, tissue lysate, a549 whole cell lysate, hepa whole cell lysate. It is guaranteed to work for WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-01
Question
I was wanting to use your anti-PKB Alpha antibody (Monoclonal, PKB-175) for WB for chicken muscle ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for chicken muscle ovary identification?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
You can see on the product datasheet, MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) has been validated for WB on chicken, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in chicken muscle ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
Our team were happy with the WB result of your anti-PKB Alpha antibody (Monoclonal, PKB-175). However we have been able to see positive staining in muscle ovary cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
From literature, muscle ovary does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-24
Question
We are interested in to test anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 on chicken muscle ovary for research purposes, then I may be interested in using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
Will MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Miller
Verified customer
Asked: 2019-10-31
Answer
As indicated on the product datasheet, MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-31
Question
I have attached the WB image, lot number and protocol we used for muscle ovary using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-07
Question
We have been able to see staining in chicken foreskin. Are there any suggestions? Is anti-PKB Alpha antibody (Monoclonal, PKB-175) supposed to stain foreskin positively?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
From what I have seen in literature foreskin does express AKT1. From what I have seen in Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-22
Question
Do you have a BSA free version of anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 available?
Verified Customer
Verified customer
Asked: 2019-04-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-04
Question
Is this MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) reactive to the isotypes of AKT1?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
The immunogen of MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) is Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-22
Question
Will anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 work for WB with muscle ovary?
A. Krishna
Verified customer
Asked: 2018-04-11
Answer
According to the expression profile of muscle ovary, AKT1 is highly expressed in muscle ovary. So, it is likely that anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 will work for WB with muscle ovary.
Boster Scientific Support
Answered: 2018-04-11
Question
Is a blocking peptide available for product anti-PKB Alpha antibody (Monoclonal, PKB-175) (MA1085)?
C. Thomas
Verified customer
Asked: 2017-10-12
Answer
We do provide the blocking peptide for product anti-PKB Alpha antibody (Monoclonal, PKB-175) (MA1085). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-12
Question
We are currently using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
B. Bhatt
Verified customer
Asked: 2016-09-15
Answer
The anti-PKB Alpha antibody (Monoclonal, PKB-175) (MA1085) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-15
Question
I see that the anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 works with WB, what is the protocol used to produce the result images on the product page?
R. Huang
Verified customer
Asked: 2016-06-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-06-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle ovary using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085. Let me know if you need anything else.
R. Brown
Verified customer
Asked: 2015-07-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-28
Question
I have a question about product MA1085, anti-PKB Alpha antibody (Monoclonal, PKB-175). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Kulkarni
Verified customer
Asked: 2013-12-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-12
Question
Does anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 work on canine WB with cervix carcinoma erythroleukemia?
M. Rodriguez
Verified customer
Asked: 2013-01-02
Answer
Our lab technicians have not validated anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-02
