Product Info Summary
SKU: | PB9184 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GST3/GST pi/GSTP1 Antibody Picoband®
View all Glutathione S-Transferase pi 1/GSTP1 Antibodies
SKU/Catalog Number
PB9184
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GST3/GST pi/GSTP1 Antibody Picoband® catalog # PB9184. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GST3/GST pi/GSTP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9184)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GST3 recombinant protein (Position: P2-Q210). Human GST3 shares 85% and 86% amino acid (aa) sequences identity with mouse and rat GST3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9184 is reactive to GSTP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23356 MW
Background of Glutathione S-Transferase pi 1/GSTP1
Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9184 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, mouse liver tissue
IHC: rat lung tissue, human lung cancer tissue
ICC/IF: SH-SY5Y cell
FCM: THP-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3/GST pi antigen affinity purified polyclonal antibody (Catalog # PB9184) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST3/GST pi at approximately 23 kDa. The expected band size for GST3/GST pi is at 23 kDa.
Click image to see more details
Figure 5. Flow Cytometry analysis of THP-1 cells using anti-GST3/GST pi antibody (PB9184).
Overlay histogram showing THP-1 cells stained with PB9184 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GST3/GST pi Antibody (PB9184, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 2. IHC analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184).
GST3/GST pi was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GST3/GST pi Antibody (PB9184) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184).
GST3/GST pi was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GST3/GST pi Antibody (PB9184) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184).
GST3/GST pi was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GST3/GST pi Antibody (PB9184) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For GSTP1 (Source: Uniprot.org, NCBI)
Gene Name
GSTP1
Full Name
Glutathione S-transferase P
Weight
23356 MW
Superfamily
GST superfamily
Alternative Names
Glutathione S-transferase P;2.5.1.18;GST class-pi;GSTP1-1;GSTP1;FAEES3, GST3; GSTP1 DFN7, FAEES3, GST3, GSTP, HEL-S-22, PI glutathione S-transferase pi 1 glutathione S-transferase P|GST class-pi|GSTP1-1|deafness, X-linked 7|epididymis secretory protein Li 22|fatty acid ethyl ester synthase III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSTP1, check out the GSTP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSTP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GST3/GST pi/GSTP1 Antibody Picoband® (PB9184)
Hello CJ!
PB9184 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Herbal Constituents in An-Gong-Niu-Huang Wan (AGNH) Protect against Cinnabar- and Realgar-Induced Hepatorenal Toxicity and Accumulations of Mercury and Arsenic in Mice
Toxicological risks of Rhizoma paridis saponins in rats involved NF-κB and Nrf2 signaling
The recovery trajectory of adolescent social defeat stress-induced behavioral, 1 H-MRS metabolites and myelin changes in Balb/c mice
Overdose Intake of Curcumin Initiates the Unbalanced State of Bodies
Ligustrazine derivate DLJ14 reduces multidrug resistance of K562/A02 cells by modulating GSTπ activity
Quetiapine mitigates the neuroinflammation and oligodendrocyte loss in the brain of C57BL/6 mouse following cuprizone exposure for one week
Glutathione S-Transferases and Cytochrome P450 enzyme expression in patients with Intracranial Tumors: Preliminary Report of 55 Patients
Enhanced expression of stem cell markers and drug resistance in sphere-forming non-small cell lung cancer cells.
The recovery trajectory of adolescent social defeat stress-induced behavioral, (1)H-MRS metabolites and myelin changes in Balb/c mice
Yang P, Gao Z, Zhang H, Fang Z, Wu C, Xu H, Huang Qj. Neuropsychiatr Dis Treat. 2015 Mar 6;11:597-607. Doi: 10.2147/Ndt.S78131. Ecollection 2015. Changes In Proinflammatory Cytokines And White Matter In Chronically Stressed Rats.
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Customer Q&As
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1 Customer Q&As for Anti-GST3/GST pi/GSTP1 Antibody Picoband®
Question
We are currently using anti-GST3/GST pi/GSTP1 antibody PB9184 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-GST3/GST pi/GSTP1 antibody (PB9184) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15