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Product Info Summary
| SKU: | P05439 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Phospho TIF-IA (Ser649) Antibody
SKU/Catalog Number
P05439
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho TIF-IA (Ser649) Antibody (Catalog# P05439). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho TIF-IA (Ser649) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05439)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TIF-IA around the phosphorylation site of Ser649. Immunogen sequence location: 602-651
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P05439 is reactive to RRN3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
74107 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P05439 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells treated with starved 24h, using TIF-IA (Phospho-Ser649) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For RRN3 (Source: Uniprot.org, NCBI)
Gene Name
RRN3
Full Name
RNA polymerase I-specific transcription initiation factor RRN3
Weight
74107 MW
Superfamily
RRN3 family
Alternative Names
RRN3;TIFIA;RNA polymerase I-specific transcription initiation factor RRN3;Transcription initiation factor IA;TIF-IA RRN3 A-270G1.2, TIFIA RRN3 homolog, RNA polymerase I transcription factor RNA polymerase I-specific transcription initiation factor RRN3|RRN3 RNA polymerase I transcription factor homolog|TIF-IA|transcription initiation factor IA|transcription initiation factor TIF-IA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRN3, check out the RRN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho TIF-IA (Ser649) Antibody (P05439)
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Customer Q&As
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3 Customer Q&As for Anti-Phospho TIF-IA (Ser649) Antibody
Question
Does P05439 anti-Phospho TIF-IA (Ser649) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
You can see on the product datasheet, P05439 anti-Phospho TIF-IA (Ser649) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-27
Question
We are currently using anti-Phospho TIF-IA (Ser649) antibody P05439 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
The anti-Phospho TIF-IA (Ser649) antibody (P05439) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-28
Question
Is this P05439 anti-Phospho TIF-IA (Ser649) antibody reactive to the isotypes of RRN3?
L. Krishna
Verified customer
Asked: 2016-10-12
Answer
The immunogen of P05439 anti-Phospho TIF-IA (Ser649) antibody is A peptide derived from human TIF-IA around the phosphorylation site of Ser649. Immunogen sequence location: 602-651. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-10-12
