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Product Info Summary
| SKU: | A04865 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MZF-1 Antibody
SKU/Catalog Number
A04865
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MZF-1 Antibody (Catalog # A04865). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MZF-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04865)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human MZF-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04865 is reactive to MZF1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
82055 MW
Background of MZF1
Binds to target promoter DNA and functions as trancription regulator. Regulates transcription from the PADI1 and CDH2 promoter. May be one regulator of transcriptional events during hemopoietic development.
Hromas R., J. Biol. Chem. 266:14183-14187(1991).
Peterson M.J., Gene 254:105-118(2000).
Olsen J.V., Cell 127:635-648(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04865 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells and K562 cells, using MZF-1 antibody A04865.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MZF-1 antibody A04865.
Protein Target Info & Infographic
Gene/Protein Information For MZF1 (Source: Uniprot.org, NCBI)
Gene Name
MZF1
Full Name
Myeloid zinc finger 1
Weight
82055 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Myeloid zinc finger 1;MZF-1;Zinc finger and SCAN domain-containing protein 6;Zinc finger protein 42;MZF1;MZF, ZNF42, ZSCAN6; MZF1 MZF-1B, ZFP98, ZNF42, ZSCAN6, MZF1 myeloid zinc finger 1 myeloid zinc finger 1|zinc finger and SCAN domain-containing protein 6|zinc finger protein 42 (myeloid-specific retinoic acid-responsive)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MZF1, check out the MZF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MZF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MZF-1 Antibody (A04865)
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Customer Q&As
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13 Customer Q&As for Anti-MZF-1 Antibody
Question
I see that the anti-MZF-1 antibody A04865 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right uterine tube using anti-MZF-1 antibody A04865. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-21
Question
Will A04865 anti-MZF-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
It shows on the product datasheet, A04865 anti-MZF-1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-MZF-1 antibody A04865 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The anti-MZF-1 antibody (A04865) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-11
Question
Does anti-MZF-1 antibody A04865 work on monkey WB with skin uterus?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Our lab technicians have not tested anti-MZF-1 antibody A04865 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-MZF-1 antibody A04865 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey skin uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-01
Question
Is there a BSA free version of anti-MZF-1 antibody A04865 available?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MZF-1 antibody A04865 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-21
Question
My question regarding product A04865, anti-MZF-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04865 anti-MZF-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-07
Question
Will anti-MZF-1 antibody A04865 work for IHC with right uterine tube?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
According to the expression profile of right uterine tube, MZF1 is highly expressed in right uterine tube. So, it is likely that anti-MZF-1 antibody A04865 will work for IHC with right uterine tube.
Boster Scientific Support
Answered: 2018-10-11
Question
I have attached the WB image, lot number and protocol we used for right uterine tube using anti-MZF-1 antibody A04865. Please let me know if you require anything else.
D. Huang
Verified customer
Asked: 2017-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-13
Question
Is a blocking peptide available for product anti-MZF-1 antibody (A04865)?
P. Li
Verified customer
Asked: 2016-07-22
Answer
We do provide the blocking peptide for product anti-MZF-1 antibody (A04865). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-07-22
Question
I was wanting to use your anti-MZF-1 antibody for IHC for human right uterine tube on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right uterine tube identification?
A. Brown
Verified customer
Asked: 2014-11-25
Answer
It shows on the product datasheet, A04865 anti-MZF-1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right uterine tube in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-25
Question
My lab would like to test anti-MZF-1 antibody A04865 on human right uterine tube for research purposes, then I may be interested in using anti-MZF-1 antibody A04865 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Krishna
Verified customer
Asked: 2014-01-30
Answer
The products we sell, including anti-MZF-1 antibody A04865, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-01-30
Question
Is this A04865 anti-MZF-1 antibody reactive to the isotypes of MZF1?
J. Krishna
Verified customer
Asked: 2013-01-09
Answer
The immunogen of A04865 anti-MZF-1 antibody is Synthesized peptide derived from N-terminal of human MZF-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-09
