Product Info Summary
SKU: | A01917S71-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-TH (S71) Antibody
View all Tyrosine Hydroxylase Antibodies
SKU/Catalog Number
A01917S71-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-TH (S71) Antibody catalog # A01917S71-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-TH (S71) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01917S71-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human TH around the phosphorylation site of S71.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01917S71-1 is reactive to TH in Human, Mouse, Rat
Applications
A01917S71-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58600 MW
Background of Tyrosine Hydroxylase
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Song B.J., Submitted (APR-1990).
Bentley D.R., Nature 434:325-337(2005).
Comb M.J., Cell 131:1190-1203(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
![a01917s71 1 anti phospho th s71 antibody ihc testing 3 a01917s71 1 anti phospho th s71 antibody ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a01917s71-1-anti-phospho-th-s71-antibody-ihc-testing-3.jpg)
Click image to see more details
Figure 3. Immunohistochemistry validation of TH using Anti-Phospho-TH (S71) Antibody (A01917S71-1).
Immunohistochemical analysis of paraffin-embedded Human brain using Anti-Phospho-TH (S71) polyclonal antibody. Antibody was diluted at 1:100 (4°C![a01917s71 1 anti phospho th s71 antibody ihc testing 4 a01917s71 1 anti phospho th s71 antibody ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a01917s71-1-anti-phospho-th-s71-antibody-ihc-testing-4.jpg)
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Figure 4. Immunohistochemistry validation of TH using Anti-Phospho-TH (S71) Antibody (A01917S71-1).
Immunohistochemical analysis of paraffin-embedded Human breast cancer using Anti-Phospho-TH (S71) polyclonal antibody. Antibody was diluted at 1:100 (4°C![a01917s71 1 anti phospho th s71 antibody wb testing 1 a01917s71 1 anti phospho th s71 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01917s71-1-anti-phospho-th-s71-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-TH (S71) Antibody A01917S71-1
Western Blot (WB) analysis of HepG2 cells using Anti-Phospho-TH (S71) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
![a01917s71 1 anti phospho th s71 antibody wb testing 2 a01917s71 1 anti phospho th s71 antibody wb testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01917s71-1-anti-phospho-th-s71-antibody-wb-testing-2.jpg)
Click image to see more details
Figure 2. Western blotting validation for Anti-Phospho-TH (S71) Antibody A01917S71-1
Western Blot (WB) analysis of Mouse Kidney lysis using Anti-Phospho-TH (S71) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TH (Source: Uniprot.org, NCBI)
Gene Name
TH
Full Name
Tyrosine 3-monooxygenase
Weight
58600 MW
Superfamily
biopterin-dependent aromatic amino acid hydroxylase family
Alternative Names
TH; DYT14; DYT5b; EC 1.14.16; EC 1.14.16.2; TH mouse; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; Tyrosine Hydroxylase immunohistochemistry; Tyrosine Hydroxylase mouse; Tyrosine Hydroxylase TH DYT14, DYT5b, TYH tyrosine hydroxylase tyrosine 3-monooxygenase|dystonia 14|tyrosine 3-hydroxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TH, check out the TH Infographic
![TH infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-TH (S71) Antibody (A01917S71-1)
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