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Product Info Summary
| SKU: | M01187-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-NGFR Antibody (C-term)
View all NGFR/TNFRSF16/p75NTR Antibodies
SKU/Catalog Number
M01187-2
Size
50 µl
Description
Boster Bio Anti-NGFR Antibody (C-term) (Catalog # M01187-2). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NGFR Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01187-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NGFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 276-310 amino acids from the C-terminal region of human NGFR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01187-2 is reactive to NGFR in Human
Reconstitution
Calculated molecular weight
45183 Da
Background of NGFR/TNFRSF16/p75NTR
Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01187-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Anti-NGFR Antibody (C-term) at 1:2000 dilution + SW480 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing SH-SY5Y cells stained with M01187-2 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01187-2, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For NGFR (Source: Uniprot.org, NCBI)
Gene Name
NGFR
Full Name
Tumor necrosis factor receptor superfamily member 16
Weight
45183 Da
Alternative Names
Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, p75 ICD, CD271, NGFR, TNFRSF16 NGFR CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR nerve growth factor receptor tumor necrosis factor receptor superfamily member 16|NGF receptor|TNFR superfamily, member 16|low affinity neurotrophin receptor p75NTR|low-affinity nerve growth factor receptor|p75 ICD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NGFR, check out the NGFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NGFR Antibody (C-term) (M01187-2)
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