Anti-P2X2/P2RX2 Antibody Picoband®

Product Name

Anti-P2X2/P2RX2 Antibody Picoband®

View all P2X2/P2RX2 Antibodies

SKU/Catalog Number

PB9303

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-P2X2/P2RX2 Antibody Picoband® catalog # PB9303. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-P2X2/P2RX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9303)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human P2X2 recombinant protein (Position: D139-L471). Human P2X2 shares 84% amino acid (aa) sequence identity with both mouse and rat P2X2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9303 is reactive to P2RX2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

60 kDa

Calculated molecular weight

51754 MW

Background of P2X2/P2RX2

The P2RX2 gene encodes the P2X2 receptor, which assembles as a trimer to form a ligand-gated ion channel gated by extracellular ATP. P2X2 receptors mediate a variety of cellular responses, including excitatory postsynaptic responses in sensory neurons. The product of this gene belongs to the family of purinoceptors for ATP. P2RX2 is mapped to 12q24.33. It has been found that ATP-activated P2RX2 influenced OHC electromotility, a stimulus-induced change in hair cell length that functions as an amplifier to determine hearing sensitivity and frequency selectivity. What’s more, P2RX2 channels were necessary for development of the temporary threshold shift.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9303 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: Rat Brain Tissue, Mouse Brain Tissue, Human Placenta Tissue, HELA Whole Cell, SHG Whole Cell, NEURO Whole Cell, 22RV1 Whole Cell, U87 Whole Cell
IHC: Human Lung Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For P2RX2 (Source: Uniprot.org, NCBI)

Alternative Names

P2X purinoceptor 2;P2X2;ATP receptor;Purinergic receptor;P2RX2;P2X2; P2RX2 DFNA41, P2X2 purinergic receptor P2X 2 P2X purinoceptor 2|ATP receptor|P2X Receptor, subunit 2|purinergic receptor P2X, ligand gated ion channel, 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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