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Product Info Summary
| SKU: | P00097-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody
SKU/Catalog Number
P00097-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody catalog # P00097-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00097-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FCO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Tau (S396)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00097-2 is reactive to MAPT in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
50-70 kDa
Calculated molecular weight
78928 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00097-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
IHC 1:100-1:500
Positive Control
IHC: human breast carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:4k dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-Tau (S396) expression in (1) SH-SY5Y cell lysate treated with AP; (2) SH-SY5Y cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tau (S396) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MAPT (Source: Uniprot.org, NCBI)
Gene Name
MAPT
Full Name
Microtubule-associated protein tau
Weight
78928 MW
Alternative Names
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau;MAPT;MAPTL, MTBT1, TAU; MAPT DDPAC, FTDP-17L, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40, MAPT microtubule associated protein tau microtubule-associated protein tau|G protein beta1/gamma2 subunit-interacting factor 1|PHF-tau|neurofibrillary tangle protein|paired helical filament-tau|protein phosphatase 1, regulatory subunit 103
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPT, check out the MAPT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody (P00097-2)
Hello CJ!
P00097-2 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang YW,Li XQ,Tan WF,Fang B,Ma H.Postoperative 24-h Acute Sleep Deprivation Improves Learning and Memory Through Inhibition of Tau Phosphorylation in the Hippocampal Neurons of Splenectomized Rats.Nat Sci Sleep.2020 Aug 24;12:603-613.doi:10.2147/NSS.S254
Species: Rat
P00097-2 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100
Micro-RNA-137 Inhibits Tau Hyperphosphorylation in Alzheimer's Disease and Targets the CACNA1C Gene in Transgenic Mice and Human Neuroblastoma %u2026
The expression of insulin-like growth factor-1 in senior patients with diabetes and dementia
Panax notoginsenoside Rb1 ameliorates Alzheimer?s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression
Chen C, Gu J, Basurto-Islas G, Jin N, Wu F, Gong CX, Iqbal K, Liu F. Sci Rep. 2017 Oct 18;7(1):13478. doi: 10.1038/s41598-017-13791-5. Up-regulation of casein kinase 1ε is involved in tau pathogenesis in Alzheimer’s disease
Han F1, Zhuang TT1, Chen JJ1, Zhu XL1,2, Cai YF1, Lu YP1. PLoS One. 2017 Sep 21;12(9):e0185102. doi: 10.1371/journal.pone.0185102. eCollection 2017. Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal ...
Li X, Li M, Li Y, Quan Q, Wang J. Neural Regen Res. 2012 Dec 25;7(36):2860-6. Doi: 10.3969/J.Issn.1673-5374.2012.36.002. Cellular And Molecular Mechanisms Underlying The Action Of Ginsenoside Rg1 Against Alzheimer'S Disease.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Tau (S396) MAPT Rabbit Monoclonal Antibody
Question
We have observed staining in rat brain. Are there any suggestions? Is anti-Phospho-Tau (S396) Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
From what I have seen in literature brain does express MAPT. From what I have seen in Uniprot.org, MAPT is expressed in parietal lobe, brain, fetal brain, fetal brain cortex, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MAPT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1512244, 2484340, 2495000, 2498079, 3131773, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 2516729
Fetal brain cortex, Pubmed ID: 16443603
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-Phospho-Tau (S396) Rabbit Monoclonal antibody P00097-2 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
B. Moore
Verified customer
Asked: 2017-08-22
Answer
The anti-Phospho-Tau (S396) Rabbit Monoclonal antibody (P00097-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-22
Question
We have tried in the past anti-Phospho-Tau (S396) Rabbit Monoclonal antibody for WB on parietal lobe a few years ago. I am using human, and We want to use the antibody for IHC next. I would like examining parietal lobe as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
I viewed the website and datasheets of our anti-Phospho-Tau (S396) Rabbit Monoclonal antibody and it seems that P00097-2 has been tested on human in both WB and IHC. Thus P00097-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-22
Question
Our team were happy with the WB result of your anti-Phospho-Tau (S396) Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell cytoplasm using this antibody. Is that expected? Could you tell me where is MAPT supposed to be expressed?
T. Jones
Verified customer
Asked: 2015-09-04
Answer
From what I have seen in literature, leukemic t-cell does express MAPT. Generally MAPT expresses in cytoplasm, cytosol. Regarding which tissues have MAPT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1512244, 2484340, 2495000, 2498079, 3131773, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 2516729
Fetal brain cortex, Pubmed ID: 16443603
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2015-09-04
