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Product Info Summary
| SKU: | A00796Y427 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Shc (Y427) SHC1 Antibody
SKU/Catalog Number
A00796Y427
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Shc (Y427) SHC1 Antibody catalog # A00796Y427. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Shc (Y427) SHC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00796Y427)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Shc around the phosphorylation site of Y427.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00796Y427 is reactive to SHC1 in Human, Mouse, Rat
Applications
A00796Y427 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
62822 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of SHC1 using Anti-Phospho-Shc (Y427) SHC1 Antibody (A00796Y427).
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C
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Figure 3. Immunohistochemistry validation of SHC1 using Anti-Phospho-Shc (Y427) SHC1 Antibody (A00796Y427).
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C
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Figure 1. Western blotting validation for Anti-Phospho-Shc (Y427) SHC1 Antibody A00796Y427
Western Blot (WB) analysis of specific cells using Phospho-Shc (Y427) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SHC1 (Source: Uniprot.org, NCBI)
Gene Name
SHC1
Full Name
SHC-transforming protein 1
Weight
62822 MW
Alternative Names
FLJ26504; p66; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC; SHC1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1 SHC1 SHC, SHCA SHC adaptor protein 1 SHC-transforming protein 1|SH2 domain protein C1|SHC (Src homology 2 domain containing) transforming protein 1|SHC-transforming protein 3|SHC-transforming protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHC1, check out the SHC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Shc (Y427) SHC1 Antibody (A00796Y427)
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