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Product Info Summary
| SKU: | P02706 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-SAPK/JNK (Thr183) MAPK9 Antibody
SKU/Catalog Number
P02706
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-SAPK/JNK (Thr183) MAPK9 Antibody (Catalog # P02706). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-SAPK/JNK (Thr183) MAPK9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02706)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Immunogen
Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02706 is reactive to MAPK9 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
48139 MW
Background of JNK2
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02706 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 46 54 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells using SAPK/JNK(Phospho-Thr183) antibody P02706(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
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Western blot analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) antibody P02706.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Phospho-Thr183) antibody P02706(left) or the same antibody preincubated with blocking peptide(right).
Protein Target Info & Infographic
Gene/Protein Information For MAPK9 (Source: Uniprot.org, NCBI)
Gene Name
MAPK9
Full Name
Mitogen-activated protein kinase 9
Weight
48139 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 9;MAP kinase 9;MAPK 9;2.7.11.24;JNK-55;Stress-activated protein kinase 1a;SAPK1a;Stress-activated protein kinase JNK2;c-Jun N-terminal kinase 2;MAPK9;JNK2, PRKM9, SAPK1A; MAPK9 JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK mitogen-activated protein kinase 9 mitogen-activated protein kinase 9|Jun kinase|MAP kinase 9|MAPK 9|c-Jun N-terminal kinase 2|c-Jun kinase 2|stress-activated protein kinase 1a|stress-activated protein kinase JNK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK9, check out the MAPK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-SAPK/JNK (Thr183) MAPK9 Antibody (P02706)
Hello CJ!
P02706 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
Species: Rat
P02706 usage in article: APP:IF, SAMPLE:ADSCS, DILUTION:1:400
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Li Y, He S, Tang J, Ding N, Chu X, Cheng L, Ding X, Liang T, Feng S, Rahman SU, Wang X, Wu J. Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway
Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, Zhang R, You H. Int J Mol Med. 2018 Apr;41(4):2117-2127. doi: 10.3892/ijmm.2018.3410. Epub 2018 Jan 22. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabol...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-SAPK/JNK (Thr183) MAPK9 Antibody
Question
I see that the anti-Phospho-SAPK/JNK (Thr183) antibody P02706 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-15
Question
Does anti-Phospho-SAPK/JNK (Thr183) antibody P02706 work on feline IHC with middle temporal gyrus?
G. Krishna
Verified customer
Asked: 2018-07-26
Answer
Our lab technicians have not validated anti-Phospho-SAPK/JNK (Thr183) antibody P02706 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Phospho-SAPK/JNK (Thr183) antibody P02706 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline middle temporal gyrus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-26
Question
We need to test anti-Phospho-SAPK/JNK (Thr183) antibody P02706 on human skin for research purposes, then I may be interested in using anti-Phospho-SAPK/JNK (Thr183) antibody P02706 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Krishna
Verified customer
Asked: 2014-10-17
Answer
The products we sell, including anti-Phospho-SAPK/JNK (Thr183) antibody P02706, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-10-17
Question
Is this P02706 anti-Phospho-SAPK/JNK (Thr183) antibody reactive to the isotypes of MAPK9?
S. Singh
Verified customer
Asked: 2013-02-25
Answer
The immunogen of P02706 anti-Phospho-SAPK/JNK (Thr183) antibody is Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-02-25
