Click image to see more details
-
-
-
-
-
+3
Product Info Summary
| SKU: | M02706 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-JNK2 MAPK9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02706
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-JNK2 MAPK9 Rabbit Monoclonal Antibody catalog # M02706. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JNK2 MAPK9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02706)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EIF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JNK2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02706 is reactive to MAPK9 in Human, Monkey, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
48 kDa
Calculated molecular weight
48139 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02706 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:30
Positive Control
WB: human Hela whole cell, human 293T whole cell, monkey COS-7 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue
IHC: Rat intestine tissue, Human prostate cancer tissue, Human astrocytoma tissue, Mouse skin tissue, human colon tissue
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:100 dilution.
Click image to see more details
Figure 1. Western blot analysis of MAPK9 using anti-MAPK9 antibody (M02706).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK9 antigen affinity purified monoclonal antibody (Catalog # M02706) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK9 at approximately 48 kDa. The expected band size for MAPK9 is at 48 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:100 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat intestine, using the Antibody at 1:100 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using JNK2 Antibody.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MAPK9 (Source: Uniprot.org, NCBI)
Gene Name
MAPK9
Full Name
Mitogen-activated protein kinase 9
Weight
48139 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 9;MAP kinase 9;MAPK 9;2.7.11.24;JNK-55;Stress-activated protein kinase 1a;SAPK1a;Stress-activated protein kinase JNK2;c-Jun N-terminal kinase 2;MAPK9;JNK2, PRKM9, SAPK1A; MAPK9 JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK mitogen-activated protein kinase 9 mitogen-activated protein kinase 9|Jun kinase|MAP kinase 9|MAPK 9|c-Jun N-terminal kinase 2|c-Jun kinase 2|stress-activated protein kinase 1a|stress-activated protein kinase JNK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK9, check out the MAPK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JNK2 MAPK9 Rabbit Monoclonal Antibody (M02706)
Hello CJ!
M02706 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
Species: Rat
M02706 usage in article: APP:IF, SAMPLE:ADSCS, DILUTION:1:400
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Li Y, He S, Tang J, Ding N, Chu X, Cheng L, Ding X, Liang T, Feng S, Rahman SU, Wang X, Wu J. Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway
Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, Zhang R, You H. Int J Mol Med. 2018 Apr;41(4):2117-2127. doi: 10.3892/ijmm.2018.3410. Epub 2018 Jan 22. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabol...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-JNK2 MAPK9 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-JNK2 MAPK9 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
