Anti-Cytokeratin 16 (S451) KRT16 Antibody

Product Name

Anti-Cytokeratin 16 (S451) KRT16 Antibody

View all Cytokeratin 16 Antibodies

SKU/Catalog Number

A03393S451

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 16 (S451) KRT16 Antibody catalog # A03393S451. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytokeratin 16 (S451) KRT16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03393S451)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Keratin 16.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03393S451 is reactive to KRT16 in Human, Mouse, Rat

Applications

A03393S451 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

51268 MW

Background of Cytokeratin 16

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.

Qian Wang, J. Virol., Jan 2004; 78: 821 - 833. RD Paladini,
RD Paladini, J. Cell Biol., Feb 1996; 132: 381.
M.K. Shamsher, Hum. Mol. Genet., Oct 1995; 4: 1875 - 1881.
Ying-Nai Wang, Mol. Pharmacol., Jan 2006; 69: 85 - 98.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of KRT16 using Anti-Cytokeratin 16 (S451) KRT16 Antibody (A03393S451).

Immunohistochemistry (IHC) analyzes of Cytokeratin 16 (S451) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-Cytokeratin 16 (S451) KRT16 Antibody A03393S451

Western blot (WB) analysis of Cytokeratin 16 (S451) polyclonal antibody at 1:500 dilution
Lane1:MCF-7 cell lysate
Lane2:sp2/0 cell lysate
Lane3:PC12 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For KRT16 (Source: Uniprot.org, NCBI)

Alternative Names

CK16; CK-16; cytokeratin 16; cytokeratin-16; FNEPPK; focal non-epidermolytic palmoplantar keratoderma; K16; K1CP; keratin 16; keratin, type I cytoskeletal 16; keratin-16; KRT16A; NEPPK KRT16 CK16, FNEPPK, K16, K1CPA, NEPPK, PC1, KRT16 keratin 16 keratin, type I cytoskeletal 16|cytokeratin 16|focal non-epidermolytic palmoplantar keratoderma|keratin 16, type I

For more info on KRT16, check out the KRT16 Infographic

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In this infographic, you will see the following information for KRT16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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