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Product Info Summary
| SKU: | M00202S232 |
|---|---|
| Size: | 100 µl/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-RIP3 (S232) Rabbit Monoclonal Antibody
View all RIPK3/RIP3 Antibodies
SKU/Catalog Number
M00202S232
Size
100 µl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-RIP3 (S232) Rabbit Monoclonal Antibody catalog # M00202S232. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-RIP3 (S232) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00202S232)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
31R36
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-RIP3 (S232)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00202S232 is reactive to RIPK3 in Mouse, Rat
Reconstitution
Observed Molecular Weight
50 kDa
Calculated molecular weight
50 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00202S232 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: rat spleen tissue, rat liver tissue, rat heart tissue, rat L6 whole cell, mouse spleen tissue, mouse liver tissue, mouse heart tissue, mouse L929 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Phospho-RIP3 (S232) using anti-Phospho-RIP3 (S232) antibody (M00202S232).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat L6 whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse L929 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-RIP3 (S232) antigen affinity purified monoclonal antibody (Catalog # M00202S232) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-RIP3 (S232) at approximately 50 kDa. The expected band size for Phospho-RIP3 (S232) is at 50 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RIPK3 (Source: Uniprot.org, NCBI)
Gene Name
RIPK3
Full Name
Receptor-interacting serine/threonine-protein kinase 3
Weight
50 kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11.1; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine-threonine kinase 3; RIP3; RIP-3; RIP3receptor interacting protein 3; RIPK3; RIP-like protein kinase 3 RIPK3 RIP3 receptor interacting serine/threonine kinase 3 receptor-interacting serine/threonine-protein kinase 3|RIP-3|RIP-like protein kinase 3|receptor interacting protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIPK3, check out the RIPK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-RIP3 (S232) Rabbit Monoclonal Antibody (M00202S232)
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