Anti-RIPK3 Antibody

Product Name

Anti-RIPK3 Antibody

View all RIPK3/RIP3 Antibodies

SKU/Catalog Number

M00202

Size

50 µl

Description

Boster Bio Anti-RIPK3 Antibody (Catalog # M00202). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RIPK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00202)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

2013CT892.86.49

Isotype

IgG1,κ

Immunogen

This RIPK3 antibody is generated from a mouse immunized with a recombinate protein from the human region of human RIPK3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00202 is reactive to RIPK3 in Human

Applications

M00202 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

56887 Da

Background of RIPK3/RIP3

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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All lanes : Anti-RIPK3 Antibody at 1:1000 dilution
Lane 1: HT-29 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: SW620 whole cell lysate
Lane 4: THP-1 whole cell lysate
Lane 5: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For RIPK3 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11.1; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine-threonine kinase 3; RIP3; RIP-3; RIP3receptor interacting protein 3; RIPK3; RIP-like protein kinase 3 RIPK3 RIP3 receptor interacting serine/threonine kinase 3 receptor-interacting serine/threonine-protein kinase 3|RIP-3|RIP-like protein kinase 3|receptor interacting protein 3

For more info on RIPK3, check out the RIPK3 Infographic

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In this infographic, you will see the following information for RIPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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