Anti-Phospho-p53 (S9) TP53 Rabbit Monoclonal Antibody

p53 antibody

Boster Bio Anti-Phospho-p53 (S9) TP53 Rabbit Monoclonal Antibody catalog # P00001-3. Tested in WB application. This antibody reacts with Human. Cited in 7 publication(s).

Product Info Summary

SKU: P00001-3
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-p53 (S9) TP53 Rabbit Monoclonal Antibody

View all p53 Antibodies

SKU/Catalog Number

P00001-3

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-p53 (S9) TP53 Rabbit Monoclonal Antibody catalog # P00001-3. Tested in WB application. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-p53 (S9) TP53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00001-3)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADO-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-p53 (S9)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00001-3 is reactive to TP53 in Human

Applications

P00001-3 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

43653 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)

Gene Name

TP53

Full Name

Cellular tumor antigen p53

Weight

43653 MW

Superfamily

p53 family

Alternative Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TP53, check out the TP53 Infographic

TP53 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P00001-3 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer

Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma

TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells

Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).

Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.

Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...

Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...

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3 Customer Q&As for Anti-Phospho-p53 (S9) TP53 Rabbit Monoclonal Antibody

Question

We are currently using anti-Phospho-p53 (S9) Rabbit Monoclonal antibody P00001-3 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

The anti-Phospho-p53 (S9) Rabbit Monoclonal antibody (P00001-3) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-22

Question

Our lab were content with the WB result of your anti-Phospho-p53 (S9) Rabbit Monoclonal antibody. However we have seen positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-10-08

Answer

From literature, kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2018-10-08

Question

We have seen staining in human blood. Are there any suggestions? Is anti-Phospho-p53 (S9) Rabbit Monoclonal antibody supposed to stain blood positively?

R. Jones

Verified customer

Asked: 2014-03-28

Answer

According to literature blood does express TP53. According to Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2014-03-28

Order DetailsPrice
P00001-3

100μL

$370
P00001-3-30ul

30μL sample

$99
P00001-3-carrier-free-1ml

Carrier Free

$2590

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