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Product Info Summary
| SKU: | P04208 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-Phospho p40 phox (Thr154) NCF4 Antibody
SKU/Catalog Number
P04208
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho p40 phox (Thr154) NCF4 Antibody (Catalog# P04208). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho p40 phox (Thr154) NCF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04208)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human p40 phox around the phosphorylation site of Thr154. Immunogen sequence location: 120-169
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P04208 is reactive to NCF4 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
39032 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04208 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500-2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For NCF4 (Source: Uniprot.org, NCBI)
Gene Name
NCF4
Full Name
Neutrophil cytosol factor 4
Weight
39032 MW
Alternative Names
NCF4;SH3PXD4;Neutrophil cytosol factor 4;NCF-4;Neutrophil NADPH oxidase factor 4;SH3 and PX domain-containing protein 4;p40-phox;p40phox NCF4 CGD3, NCF, P40PHOX, SH3PXD4 neutrophil cytosolic factor 4 neutrophil cytosol factor 4|NCF-4|SH3 and PX domain-containing protein 4|neutrophil NADPH oxidase factor 4|neutrophil cytosolic factor 4, 40kDa|p40-phox
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCF4, check out the NCF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho p40 phox (Thr154) NCF4 Antibody (P04208)
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No publications found for P04208
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Customer Q&As
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3 Customer Q&As for Anti-Phospho p40 phox (Thr154) NCF4 Antibody
Question
Is a blocking peptide available for product anti-Phospho p40 phox (Thr154) antibody (P04208)?
E. Jha
Verified customer
Asked: 2018-11-30
Answer
We do provide the blocking peptide for product anti-Phospho p40 phox (Thr154) antibody (P04208). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-30
Question
We are currently using anti-Phospho p40 phox (Thr154) antibody P04208 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
S. Roberts
Verified customer
Asked: 2018-04-04
Answer
The anti-Phospho p40 phox (Thr154) antibody (P04208) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-04
Question
My question regarding product P04208, anti-Phospho p40 phox (Thr154) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Carter
Verified customer
Asked: 2015-06-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04208 anti-Phospho p40 phox (Thr154) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-15
