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Product Info Summary
| SKU: | A04208 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p40-phox (R149) NCF4 Antibody
SKU/Catalog Number
A04208
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p40-phox (R149) NCF4 Antibody catalog # A04208. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p40-phox (R149) NCF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04208)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 115-170 of Human p40-phox.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04208 is reactive to NCF4 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
39032 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04208 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of NCF4 using Anti-p40-phox (R149) NCF4 Antibody (A04208).
Immunohistochemistry (IHC) analyzes of p40-phox (R149) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-p40-phox (R149) NCF4 Antibody A04208
Western blot (WB) analysis of p40-phox (R149) pAb at 1:500 dilutionLane1:THP-1 whole cell lysate(40ug)
Lane2:K562 whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:Jurkat whole cell lysate(40ug)
Lane5:BV2 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For NCF4 (Source: Uniprot.org, NCBI)
Gene Name
NCF4
Full Name
Neutrophil cytosol factor 4
Weight
39032 MW
Alternative Names
Neutrophil cytosol factor 4;NCF-4;Neutrophil NADPH oxidase factor 4;SH3 and PX domain-containing protein 4;p40-phox;p40phox;NCF4;SH3PXD4; NCF4 CGD3, NCF, P40PHOX, SH3PXD4 neutrophil cytosolic factor 4 neutrophil cytosol factor 4|NCF-4|SH3 and PX domain-containing protein 4|neutrophil NADPH oxidase factor 4|neutrophil cytosolic factor 4, 40kDa|p40-phox
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCF4, check out the NCF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p40-phox (R149) NCF4 Antibody (A04208)
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