Anti-Phospho-NOS2 (Y151) Antibody

Product Name

Anti-Phospho-NOS2 (Y151) Antibody

View all NOS2 Antibodies

SKU/Catalog Number

A00368Y151

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-NOS2 (Y151) Antibody catalog # A00368Y151. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-NOS2 (Y151) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00368Y151)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human NOS2 around the phosphorylation site of Y151.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with eNOS or nNOS.

Reactive Species

A00368Y151 is reactive to NOS2 in Human, Mouse, Rat

Applications

A00368Y151 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

131117 MW

Background of NOS2

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781.
Eugene Estella, Diabetes, Aug 2006; 55: 2212 - 2219.
Yong Gu, J. Biol. Chem., May 1996; 271: 10816.
Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:100-1:300
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 1. Immunohistochemistry validation of NOS2 using Anti-Phospho-NOS2 (Y151) Antibody (A00368Y151).

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C

Gene/Protein Information For NOS2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.14.13.39; Hepatocyte NOS; HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2; Inducible NO synthase; Inducible NOS; iNOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS; NOS, type II; NOS2; NOS2A; NOS2ANOS type II NOS2 HEP-NOS, INOS, NOSA, NOS2 nitric oxide synthase 2 nitric oxide synthase, inducible|NOS, type II|hepatocyte NOS|inducible NO synthase|inducible NOS|nitric oxide synthase 2, inducible|nitric oxide synthase 2A (inducible, hepatocytes)|nitric oxide synthase, macrophage|peptidyl-cysteine S-nitrosylase NOS2

For more info on NOS2, check out the NOS2 Infographic

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In this infographic, you will see the following information for NOS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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