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Product Info Summary
| SKU: | P01799 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-MEF-2D (Ser444) Antibody
SKU/Catalog Number
P01799
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-MEF-2D (Ser444) Antibody (Catalog # P01799). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MEF-2D (Ser444) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01799)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MEF-2D around the phosphorylation site of S444.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01799 is reactive to MEF2D in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
55938 MW
Background of MEF2D
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253.
Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123.
Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01799 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
ELISA: 1/10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of K562 AD293 using Phospho-MEF-2D (S444) antibody.
Protein Target Info & Infographic
Gene/Protein Information For MEF2D (Source: Uniprot.org, NCBI)
Gene Name
MEF2D
Full Name
Myocyte-specific enhancer factor 2D
Weight
55938 MW
Superfamily
MEF2 family
Alternative Names
Myocyte-specific enhancer factor 2D;MEF2D; MEF2D myocyte enhancer factor 2D myocyte-specific enhancer factor 2D|MADS box transcription enhancer factor 2, polypeptide D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MEF2D, check out the MEF2D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MEF2D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MEF-2D (Ser444) Antibody (P01799)
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No publications found for P01799
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-MEF-2D (Ser444) Antibody
Question
Does P01799 anti-Phospho-MEF-2D (Ser444) Polyclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
It shows on the product datasheet, P01799 anti-Phospho-MEF-2D (Ser444) Polyclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-12
Question
We are currently using anti-Phospho-MEF-2D (Ser444) Polyclonal antibody P01799 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
The anti-Phospho-MEF-2D (Ser444) Polyclonal antibody (P01799) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-22
Question
Is a blocking peptide available for product anti-Phospho-MEF-2D (Ser444) Polyclonal antibody (P01799)?
S. Williams
Verified customer
Asked: 2015-09-07
Answer
We do provide the blocking peptide for product anti-Phospho-MEF-2D (Ser444) Polyclonal antibody (P01799). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-09-07
Question
Will anti-Phospho-MEF-2D (Ser444) Polyclonal antibody P01799 work for WB with eye testis?
V. Miller
Verified customer
Asked: 2014-09-25
Answer
According to the expression profile of eye testis, MEF2D is highly expressed in eye testis. So, it is likely that anti-Phospho-MEF-2D (Ser444) Polyclonal antibody P01799 will work for WB with eye testis.
Boster Scientific Support
Answered: 2014-09-25
Question
My lab would like to test anti-Phospho-MEF-2D (Ser444) Polyclonal antibody P01799 on rat eye testis for research purposes, then I may be interested in using anti-Phospho-MEF-2D (Ser444) Polyclonal antibody P01799 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Miller
Verified customer
Asked: 2013-01-30
Answer
The products we sell, including anti-Phospho-MEF-2D (Ser444) Polyclonal antibody P01799, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-01-30
