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Product Info Summary
| SKU: | P00409 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-KAP1 (S824) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00409
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-KAP1 (S824) Rabbit Monoclonal Antibody catalog # P00409. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-KAP1 (S824) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00409)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22T44
Isotype
IgG
Immunogen
A synthesized peptide derived from human KAP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00409 is reactive to TRIM28 in Human
Reconstitution
Observed Molecular Weight
100 kDa
Calculated molecular weight
43439 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00409 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human HEL whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KAP1 using anti-KAP1 antibody (P00409).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAP1 antigen affinity purified monoclonal antibody (Catalog # P00409) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAP1 at approximately 100 kDa. The expected band size for KAP1 is at 89 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TRIM28 (Source: Uniprot.org, NCBI)
Gene Name
TRIM28
Full Name
Transcription intermediary factor 1-beta
Weight
43439 MW
Superfamily
TRIM/RBCC family
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;2.7.12.2;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K1;MEK1, PRKMK1; TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B tripartite motif containing 28 transcription intermediary factor 1-beta|E3 SUMO-protein ligase TRIM28|KAP-1|KRAB [Kruppel-associated box domain]-associated protein 1|KRAB-interacting protein 1|KRIP-1|RING finger protein 96|RING-type E3 ubiquitin transferase TIF1-beta|TIF1-beta|nuclear corepressor KAP-1|protein phosphatase 1, regulatory subunit 157|transcriptional intermediary factor 1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM28, check out the TRIM28 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-KAP1 (S824) Rabbit Monoclonal Antibody (P00409)
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