Anti-TRIM28 Antibody

Product Name

Anti-TRIM28 Antibody

View all KAP1 Antibodies

SKU/Catalog Number

A00409

Size

100ul

Form

Liquid

Description

Boster Bio Anti-TRIM28 Antibody catalog # A00409. Tested in WB,IHC,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRIM28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00409)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human GATA-1 around the phosphorylation site of S310.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with eNOS or nNOS.

Reactive Species

A00409 is reactive to TRIM28 in Human, Mouse, Rat

Applications

A00409 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

88550 MW

Background of KAP1

Isoform 1:Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

Kan-Tai Hsia, Development, Jan 2003; 130: 369.
Jim Selfridge, Nucleic Acids Res., Nov 2001; 29: 4541 - 4550.
Cha-Kyung Youn, Cancer Res., Jul 2004; 64: 4849 - 4857.
Teresa A. Motycka, J. Biol. Chem., Apr 2004; 279: 13634 - 13639.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000-1:3000
IHC: 1:100-1:200
ICC/IF: 1:50-1:200
IP: 1:50-1:200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For TRIM28 (Source: Uniprot.org, NCBI)

Alternative Names

E3 SUMO-protein ligase TRIM28; EC 6.3.2.-; FLJ29029; KAP1; KAP-1; KAP1KRAB-associated protein 1; KRIP-1; Nuclear corepressor KAP-1; RNF96; RNF96KRAB-interacting protein 1; TF1B; TIF1B; TIF1-beta; TIF1BRING finger protein 96; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; TRIM28; tripartite motif containing 28; tripartite motif-containing 28; Tripartite motif-containing protein 28 TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B tripartite motif containing 28 transcription intermediary factor 1-beta|E3 SUMO-protein ligase TRIM28|KAP-1|KRAB [Kruppel-associated box domain]-associated protein 1|KRAB-interacting protein 1|KRIP-1|RING finger protein 96|RING-type E3 ubiquitin transferase TIF1-beta|TIF1-beta|nuclear corepressor KAP-1|protein phosphatase 1, regulatory subunit 157|transcriptional intermediary factor 1-beta

For more info on TRIM28, check out the TRIM28 Infographic

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In this infographic, you will see the following information for TRIM28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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