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Product Info Summary
| SKU: | M00409 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-KAP1 TRIM28 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00409
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KAP1 TRIM28 Rabbit Monoclonal Antibody catalog # M00409. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAP1 TRIM28 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00409)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BOE-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KAP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00409 is reactive to TRIM28 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
88550 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00409 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of KAP1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TRIM28 (Source: Uniprot.org, NCBI)
Gene Name
TRIM28
Full Name
Transcription intermediary factor 1-beta
Weight
88550 MW
Superfamily
TRIM/RBCC family
Alternative Names
Transcription intermediary factor 1-beta;TIF1-beta;E3 SUMO-protein ligase TRIM28;6.3.2.-;KRAB-associated protein 1;KAP-1;KRAB-interacting protein 1;KRIP-1;Nuclear corepressor KAP-1;RING finger protein 96;Tripartite motif-containing protein 28;TRIM28;KAP1, RNF96, TIF1B; TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B tripartite motif containing 28 transcription intermediary factor 1-beta|E3 SUMO-protein ligase TRIM28|KAP-1|KRAB [Kruppel-associated box domain]-associated protein 1|KRAB-interacting protein 1|KRIP-1|RING finger protein 96|RING-type E3 ubiquitin transferase TIF1-beta|TIF1-beta|nuclear corepressor KAP-1|protein phosphatase 1, regulatory subunit 157|transcriptional intermediary factor 1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM28, check out the TRIM28 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KAP1 TRIM28 Rabbit Monoclonal Antibody (M00409)
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No publications found for M00409
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-KAP1 TRIM28 Rabbit Monoclonal Antibody
Question
Our lab want to know about to test anti-KAP1 Rabbit Monoclonal antibody M00409 on human ovarian carcinoma for research purposes, then I may be interested in using anti-KAP1 Rabbit Monoclonal antibody M00409 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Johnson
Verified customer
Asked: 2020-05-01
Answer
The products we sell, including anti-KAP1 Rabbit Monoclonal antibody M00409, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-01
Question
My question regarding product M00409, anti-KAP1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00409 anti-KAP1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-29
Question
Is a blocking peptide available for product anti-KAP1 Rabbit Monoclonal antibody (M00409)?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We do provide the blocking peptide for product anti-KAP1 Rabbit Monoclonal antibody (M00409). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-28
Question
Would anti-KAP1 Rabbit Monoclonal antibody M00409 work for WB with ovarian carcinoma?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
According to the expression profile of ovarian carcinoma, TRIM28 is highly expressed in ovarian carcinoma. So, it is likely that anti-KAP1 Rabbit Monoclonal antibody M00409 will work for WB with ovarian carcinoma.
Boster Scientific Support
Answered: 2020-02-26
Question
We want using your anti-KAP1 Rabbit Monoclonal antibody for positive regulation of dna binding studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We appreciate your inquiry. This M00409 anti-KAP1 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-19
Question
Do you have a BSA free version of anti-KAP1 Rabbit Monoclonal antibody M00409 available?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KAP1 Rabbit Monoclonal antibody M00409 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-07
Question
I see that the anti-KAP1 Rabbit Monoclonal antibody M00409 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-03
Question
We ordered your anti-KAP1 Rabbit Monoclonal antibody for IF on testis in the past. I am using mouse, and We want to use the antibody for WB next. Our lab want to know about examining testis as well as ovarian carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
I looked at the website and datasheets of our anti-KAP1 Rabbit Monoclonal antibody and it appears that M00409 has been tested on mouse in both IF and WB. Thus M00409 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-20
Question
I was wanting to use your anti-KAP1 Rabbit Monoclonal antibody for WB for human ovarian carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
It shows on the product datasheet, M00409 anti-KAP1 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-08
Question
My team were well pleased with the WB result of your anti-KAP1 Rabbit Monoclonal antibody. However we have been able to see positive staining in liver nucleus using this antibody. Is that expected? Could you tell me where is TRIM28 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Based on literature, liver does express TRIM28. Generally TRIM28 expresses in nucleus. Regarding which tissues have TRIM28 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Ovarian carcinoma, Pubmed ID: 17512541
Skin, and Uterus, Pubmed ID: 15489334
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-05-14
Question
Will M00409 anti-KAP1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-02
Answer
You can see on the product datasheet, M00409 anti-KAP1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovarian carcinoma using anti-KAP1 Rabbit Monoclonal antibody M00409. Let me know if you need anything else.
B. Gonzalez
Verified customer
Asked: 2018-08-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-24
Question
See below the WB image, lot number and protocol we used for ovarian carcinoma using anti-KAP1 Rabbit Monoclonal antibody M00409. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-02
Question
We are currently using anti-KAP1 Rabbit Monoclonal antibody M00409 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
The anti-KAP1 Rabbit Monoclonal antibody (M00409) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-31
Question
Is this M00409 anti-KAP1 Rabbit Monoclonal antibody reactive to the isotypes of TRIM28?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
The immunogen of M00409 anti-KAP1 Rabbit Monoclonal antibody is A synthesized peptide derived from human KAP1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-25
Question
We have seen staining in mouse testis. What should we do? Is anti-KAP1 Rabbit Monoclonal antibody supposed to stain testis positively?
B. Mitchell
Verified customer
Asked: 2013-09-06
Answer
From literature testis does express TRIM28. From Uniprot.org, TRIM28 is expressed in testis, spleen, skin uterus, t-cell, ovarian carcinoma, lymphoblast, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TRIM28 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Ovarian carcinoma, Pubmed ID: 17512541
Skin, and Uterus, Pubmed ID: 15489334
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2013-09-06
