Anti-JAK1 (Ab-1022) Antibody

Product Name

Anti-JAK1 (Ab-1022) Antibody

View all JAK1 Antibodies

SKU/Catalog Number

A00330-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-JAK1 (Ab-1022) Antibody (Catalog # A00330-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-JAK1 (Ab-1022) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00330-1)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa. 1020~1024 (K-E-Y-Y-T) derived from Human JAK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00330-1 is reactive to JAK1 in Human, Mouse, Rat

Applications

A00330-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

65336 MW

Background of JAK1

Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Wang R, et al. (2003) Arch Biochem Biophys. 410(1): 7-15.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 130kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of JAK1 using anti-JAK1 antibody (A00330-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK1 antigen affinity purified polyclonal antibody (Catalog # A00330-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for JAK1.

Click image to see more details

Figure 2. IHC analysis of JAK1 using anti-JAK1 antibody (A00330-1).
JAK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-JAK1 Antibody (A00330-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For JAK1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.10; Jak1; JAK-1; JAK1Atyrosine-protein kinase JAK1; JAK1B; Janus kinase 1EC 2.7.10.2; JTK3 JAK1 AIIDEA, JAK1B, JTK3, JAK1 Janus kinase 1 tyrosine-protein kinase JAK1

For more info on JAK1, check out the JAK1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for JAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the JAK1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis