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Product Info Summary
| SKU: | A00330-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-JAK1 (Ab-1022) Antibody
SKU/Catalog Number
A00330-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-JAK1 (Ab-1022) Antibody (Catalog # A00330-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JAK1 (Ab-1022) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00330-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 1020~1024 (K-E-Y-Y-T) derived from Human JAK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00330-1 is reactive to JAK1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
65336 MW
Background of JAK1
Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Wang R, et al. (2003) Arch Biochem Biophys. 410(1): 7-15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00330-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 130kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of JAK1 using anti-JAK1 antibody (A00330-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK1 antigen affinity purified polyclonal antibody (Catalog # A00330-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for JAK1.
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Figure 2. IHC analysis of JAK1 using anti-JAK1 antibody (A00330-1).
JAK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-JAK1 Antibody (A00330-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For JAK1 (Source: Uniprot.org, NCBI)
Gene Name
JAK1
Full Name
Tyrosine-protein kinase JAK1
Weight
65336 MW
Superfamily
protein kinase superfamily
Alternative Names
Cytochrome b-245 heavy chain;1.-.-.-;CGD91-phox;Cytochrome b (558) subunit beta;Cytochrome b558 subunit beta;Heme-binding membrane glycoprotein gp91phox;NADPH oxidase 2;Neutrophil cytochrome b 91 kDa polypeptide;Superoxide-generating NADPH oxidase heavy chain subunit;gp91-1;gp91-phox;p22 phagocyte B-cytochrome;CYBB JAK1 AIIDEA, JAK1B, JTK3, JAK1 Janus kinase 1 tyrosine-protein kinase JAK1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK1, check out the JAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JAK1 (Ab-1022) Antibody (A00330-1)
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No publications found for A00330-1
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Customer Q&As
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7 Customer Q&As for Anti-JAK1 (Ab-1022) Antibody
Question
We are currently using anti-JAK1 (Ab-1022) antibody A00330-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-JAK1 (Ab-1022) antibody (A00330-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
Question
I would like to test anti-JAK1 (Ab-1022) antibody A00330-1 on human blood for research purposes, then I may be interested in using anti-JAK1 (Ab-1022) antibody A00330-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The products we sell, including anti-JAK1 (Ab-1022) antibody A00330-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-25
Question
We were content with the WB result of your anti-JAK1 (Ab-1022) antibody. However we have seen positive staining in colon tumor endomembrane system using this antibody. Is that expected? Could you tell me where is JAK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
According to literature, colon tumor does express JAK1. Generally JAK1 expresses in endomembrane system. Regarding which tissues have JAK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334, 16710414
Colon tumor, Pubmed ID: 7896447
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-01-20
Question
We have tried in the past anti-JAK1 (Ab-1022) antibody for WB on blood a few years ago. I am using human, and I plan to use the antibody for IHC next. I am looking for examining blood as well as colon tumor in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
I have checked the website and datasheets of our anti-JAK1 (Ab-1022) antibody and it seems that A00330-1 has been validated on human in both WB and IHC. Thus A00330-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-04
Question
I was wanting to use your anti-JAK1 (Ab-1022) antibody for IHC for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
You can see on the product datasheet, A00330-1 anti-JAK1 (Ab-1022) antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
We have seen staining in human brain. Do you have any suggestions? Is anti-JAK1 (Ab-1022) antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
Based on literature brain does express JAK1. Based on Uniprot.org, JAK1 is expressed in blood, brain, colon tumor, liver, among other tissues. Regarding which tissues have JAK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334, 16710414
Colon tumor, Pubmed ID: 7896447
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-12-11
Question
Is this A00330-1 anti-JAK1 (Ab-1022) antibody reactive to the isotypes of JAK1?
Verified Customer
Verified customer
Asked: 2017-09-27
Answer
The immunogen of A00330-1 anti-JAK1 (Ab-1022) antibody is Peptide sequence around aa. 1020~1024 (K-E-Y-Y-T) derived from Human JAK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-27
