Product Info Summary
SKU: | M00274-2 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-SOCS3 Mouse Monoclonal Antibody [Clone ID: OTI5D4]
SKU/Catalog Number
M00274-2
Size
100 µl
Description
Boster Bio SOCS3 mouse monoclonal antibody, clone OTI5D4 (formerly 5D4). Catalog# M00274-2. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-SOCS3 Mouse Monoclonal Antibody [Clone ID: OTI5D4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00274-2)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5D4
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human SOCS3 (NP_003946) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00274-2 is reactive to SOCS3 in Human, Mouse, Rat
Applications
M00274-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Validation Images & Assay Conditions
![m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 wb testing 1 m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m00274-2-anti-socs3-mouse-monoclonal-antibody-clone-id-oti5d4-wb-testing-1.jpg)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
![m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 wb testing 2 m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 wb testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m00274-2-anti-socs3-mouse-monoclonal-antibody-clone-id-oti5d4-wb-testing-2.jpg)
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-SOCS3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
![m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 ihc testing 3 m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 ihc testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m00274-2-anti-socs3-mouse-monoclonal-antibody-clone-id-oti5d4-ihc-testing-3.jpg)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
![m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 ihc testing 4 m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m00274-2-anti-socs3-mouse-monoclonal-antibody-clone-id-oti5d4-ihc-testing-4.jpg)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
![m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 flow cytometry testing 5 m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 flow cytometry testing 5](https://www.bosterbio.com/media/catalog/product/m/0/m00274-2-anti-socs3-mouse-monoclonal-antibody-clone-id-oti5d4-flow-cytometry-testing-5.jpg)
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HEK293T cells transfected with either SOCS3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SOCS3 antibody (M00274-2)
![m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 if testing 6 m00274 2 anti socs3 mouse monoclonal antibody clone id oti5d4 if testing 6](https://www.bosterbio.com/media/catalog/product/m/0/m00274-2-anti-socs3-mouse-monoclonal-antibody-clone-id-oti5d4-if-testing-6.jpg)
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Anti-SOCS3 mouse monoclonal antibody (M00274-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3.
Protein Target Info & Infographic
Gene/Protein Information For SOCS3 (Source: Uniprot.org, NCBI)
Gene Name
SOCS3
Full Name
Suppressor of cytokine signaling 3
Weight
Alternative Names
ATOD4; CIS3; CIS3CIS-3; Cish3; cytokine-induced SH2 protein 3; Cytokine-inducible SH2 protein 3; SOCS3; SOCS-3; SOCS-3STAT-induced STAT inhibitor 3; SSI-3; SSI-3ATOD4; SSI3MGC71791; suppressor of cytokine signaling 3 SOCS3 ATOD4, CIS3, Cish3, SOCS-3, SSI-3, SSI3 suppressor of cytokine signaling 3 suppressor of cytokine signaling 3|STAT-induced STAT inhibitor 3|cytokine-inducible SH2 protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOCS3, check out the SOCS3 Infographic
![SOCS3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOCS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOCS3 Mouse Monoclonal Antibody [Clone ID: OTI5D4] (M00274-2)
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