Product Info Summary
SKU: | A06717T17 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody
SKU/Catalog Number
A06717T17
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody catalog # A06717T17. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06717T17)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Histone H1 around the phosphorylation site of T17.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06717T17 is reactive to HIST1H1B in Human, Monkey, Mouse
Reconstitution
Calculated molecular weight
22580 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06717T17 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SKOV3 using p-Histone H1 (T17) antibody. Antibody was diluted at 1:1000.
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Western blot analysis of lysates from Jurkat cells treated with UV 15', using Histone H1 (Phospho-Thr17) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Histone H1 (Phospho-Thr17) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using Histone H1 (Phospho-Thr17) Antibody. The picture on the right is blocked with the phospho peptide.
Click image to see more details
Immunofluorescence analysis of Hela cell.
1, Histone H1 (phospho Thr17) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight).
2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 NA was diluted at 1:1000 (room temperature, 50min).
Protein Target Info & Infographic
Gene/Protein Information For HIST1H1B (Source: Uniprot.org, NCBI)
Gene Name
HIST1H1B
Full Name
Histone H1.5
Weight
22580 MW
Superfamily
histone H1/H5 family
Alternative Names
Histone H1.5;Histone H1a;Histone H1b;Histone H1s-3;HIST1H1B;H1F5; H1-5 H1, H1.5, H1B, H1F5, H1s-3, HIST1H1B H1.5 linker histone, cluster member histone H1.5|H1 histone family, member 5|histone 1, H1b|histone H1a|histone H1b|histone H1s-3|histone cluster 1 H1 family member b|histone cluster 1, H1b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H1B, check out the HIST1H1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody (A06717T17)
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