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Product Info Summary
| SKU: | A06714-2 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NF-YB Antibody
SKU/Catalog Number
A06714-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NF-YB Antibody catalog # A06714-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-YB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06714-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06714-2 is reactive to NFYB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
22831 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06714-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using NF-YB Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NFYB (Source: Uniprot.org, NCBI)
Gene Name
NFYB
Full Name
Nuclear transcription factor Y subunit beta
Weight
22831 MW
Superfamily
NFYB/HAP3 subunit family
Alternative Names
Nuclear transcription factor Y subunit beta;CAAT box DNA-binding protein subunit B;Nuclear transcription factor Y subunit B;NF-YB;NFYB;HAP3; NFYB CBF-A, CBF-B, HAP3, NF-YB nuclear transcription factor Y subunit beta nuclear transcription factor Y subunit beta|CAAT box DNA-binding protein subunit B|CCAAT-binding transcription factor subunit A|Transcription factor NF-Y, B subunit|nuclear transcription factor Y subunit B|nuclear transcription factor Y, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFYB, check out the NFYB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFYB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF-YB Antibody (A06714-2)
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Customer Q&As
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4 Customer Q&As for Anti-NF-YB Antibody
Question
Would anti-NF-YB antibody A06714-2 work on primate WB with urinary bladder?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
Our lab technicians have not tested anti-NF-YB antibody A06714-2 on primate. You can run a BLAST between primate and the immunogen sequence of anti-NF-YB antibody A06714-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate urinary bladder in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-16
Question
Would A06714-2 anti-NF-YB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Williams
Verified customer
Asked: 2019-01-07
Answer
As indicated on the product datasheet, A06714-2 anti-NF-YB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-07
Question
We are currently using anti-NF-YB antibody A06714-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
The anti-NF-YB antibody (A06714-2) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-21
Question
Will anti-NF-YB antibody A06714-2 work for WB with urinary bladder?
O. Jha
Verified customer
Asked: 2014-10-01
Answer
According to the expression profile of urinary bladder, NFYB is highly expressed in urinary bladder. So, it is likely that anti-NF-YB antibody A06714-2 will work for WB with urinary bladder.
Boster Scientific Support
Answered: 2014-10-01
