Anti-NF-YB Antibody

NFYB antibody

Boster Bio Anti-NF-YB Antibody catalog # A06714-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A06714-2
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-NF-YB Antibody

View all NFYB Antibodies

SKU/Catalog Number

A06714-2

Size

100μl

Form

Liquid

Description

Boster Bio Anti-NF-YB Antibody catalog # A06714-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NF-YB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06714-2)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the N-terminal region of human NF-YB. at AA rangle: 10-90

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06714-2 is reactive to NFYB in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

22831 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06714-2 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Positive Control

Validation Images & Assay Conditions

Gene/Protein Information For NFYB (Source: Uniprot.org, NCBI)

Gene Name

NFYB

Full Name

Nuclear transcription factor Y subunit beta

Weight

22831 MW

Superfamily

NFYB/HAP3 subunit family

Alternative Names

CAAT box DNA-binding protein subunit B; CBF-A; HAP3CBF-B; NF-YBCCAAT-binding transcription factor subunit A; Nuclear transcription factor Y subunit B; nuclear transcription factor Y subunit beta; nuclear transcription factor Y, beta; Transcription factor NF-Y, B subunit NFYB CBF-A, CBF-B, HAP3, NF-YB nuclear transcription factor Y subunit beta nuclear transcription factor Y subunit beta|CAAT box DNA-binding protein subunit B|CCAAT-binding transcription factor subunit A|Transcription factor NF-Y, B subunit|nuclear transcription factor Y subunit B|nuclear transcription factor Y, beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFYB, check out the NFYB Infographic

NFYB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFYB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-NF-YB Antibody

Question

Would anti-NF-YB antibody A06714-2 work on primate WB with urinary bladder?

Verified Customer

Verified customer

Asked: 2019-01-16

Answer

Our lab technicians have not tested anti-NF-YB antibody A06714-2 on primate. You can run a BLAST between primate and the immunogen sequence of anti-NF-YB antibody A06714-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate urinary bladder in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-16

Question

Would A06714-2 anti-NF-YB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Williams

Verified customer

Asked: 2019-01-07

Answer

As indicated on the product datasheet, A06714-2 anti-NF-YB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-07

Question

We are currently using anti-NF-YB antibody A06714-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-21

Answer

The anti-NF-YB antibody (A06714-2) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-21

Question

Will anti-NF-YB antibody A06714-2 work for WB with urinary bladder?

O. Jha

Verified customer

Asked: 2014-10-01

Answer

According to the expression profile of urinary bladder, NFYB is highly expressed in urinary bladder. So, it is likely that anti-NF-YB antibody A06714-2 will work for WB with urinary bladder.

Boster Scientific Support

Answered: 2014-10-01

Order DetailsPrice
A06714-2

100uL

$399

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A06714-2
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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