Anti-NF-Y NFYB Antibody

Product Name

Anti-NF-Y NFYB Antibody

View all NFYB Antibodies

SKU/Catalog Number

A06714

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NF-Y NFYB Antibody (Catalog # A06714). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-NF-Y NFYB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06714)

Host

Rabbit

Contents

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06714 is reactive to NFYB in Human

Reconstitution

Calculated molecular weight

22831 MW

Background of NFYB

Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A06714 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
Gel Shift: 2.0 to 4.0 µl
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

Gene/Protein Information For NFYB (Source: Uniprot.org, NCBI)

Alternative Names

NF-Y, Nuclear transcription factor Y subunit B NFYB CBF-A, CBF-B, HAP3, NF-YB nuclear transcription factor Y subunit beta nuclear transcription factor Y subunit beta|CAAT box DNA-binding protein subunit B|CCAAT-binding transcription factor subunit A|Transcription factor NF-Y, B subunit|nuclear transcription factor Y subunit B|nuclear transcription factor Y, beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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