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Product Info Summary
| SKU: | A00030Y222 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-ERK 1/2 (Y222/205) MAPK1 Antibody
SKU/Catalog Number
A00030Y222
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-ERK 1/2 (Y222/205) MAPK1 Antibody catalog # A00030Y222. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-ERK 1/2 (Y222/205) MAPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00030Y222)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human ERK 1/2 (phospho Tyr222/205)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00030Y222 is reactive to MAPK1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
41390 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00030Y222 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:50-200
IHC 1:50-300
ELISA 1:10000
Validation Images & Assay Conditions
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Immunofluorescence analysis of Hela cell. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody (red) was diluted at 1:200 (4° overnight). β-tubulin Monoclonal antibody (M7) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Immunofluorescence analysis of rat-lung tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of rat-lung tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of rat-spleen tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of rat-spleen tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Western Blot analysis of KB cells using Phospho-ERK 1/2 (Y222/205) polyclonal antibody diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, ERK 1/2 (Phospho- Tyr222/205) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Protein Target Info & Infographic
Gene/Protein Information For MAPK1 (Source: Uniprot.org, NCBI)
Gene Name
MAPK1
Full Name
Mitogen-activated protein kinase 1
Weight
41390 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 1;MAP kinase 1;MAPK 1;2.7.11.24;ERT1;Extracellular signal-regulated kinase 2;ERK-2;MAP kinase isoform p42;p42-MAPK;Mitogen-activated protein kinase 2;MAP kinase 2;MAPK 2;MAPK1;ERK2, PRKM1, PRKM2; MAPK1 ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK mitogen-activated protein kinase 1 mitogen-activated protein kinase 1|MAP kinase 1|MAP kinase 2|MAPK 2|extracellular signal-regulated kinase 2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK1, check out the MAPK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ERK 1/2 (Y222/205) MAPK1 Antibody (A00030Y222)
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